Recombinant Human KGF-1/FGF-7

Name

Recombinant Human KGF-1/FGF-7 (Recombinant Human Keratinocyte Growth Factor-1/FGF-7; rHuKGF-1/FGF-7)；重组人角质细胞生长因子1/成纤维细胞生长因子7

Synonyms

FGF7; FGF-7; FGF-7; fibroblast growth factor 7HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGFfibroblast growth factor 7 (keratinocyte growth factor)

Purity

>96% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10ng/ml, corresponding to a specific activity of >1.0×10⁵IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in 20mM PB, 0.5M NaCl, pH8.0.

Endotoxin

Less than 1EU/μg of rHuKGF-1/FGF-7 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparin sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51% amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, human KGF-1 shares 96% and 92% amino acid sequence identity with murine and rat FGF-7, respectively.

Amino Acid Sequence

CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT

产品编号

产品名称

包装

KL--5466-2μg

Recombinant Human KGF-1/FGF-7

2μg

KL-5466-10μg

Recombinant Human KGF-1/FGF-7

10μg

KL-5466-100μg

Recombinant Human KGF-1/FGF-7

100μg

KL-5466-1mg

Recombinant Human KGF-1/FGF-7

1mg

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说明书

1份