产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5466-100μg |
Recombinant Human KGF-1/FGF-7 |
100μg |
4759.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
2252
|
P21781
|
E. coli
|
163aa
|
18.9kDa
|
-
|
About this Antibody
|
Name
|
Recombinant Human KGF-1/FGF-7 (Recombinant Human Keratinocyte Growth Factor-1/FGF-7; rHuKGF-1/FGF-7);重组人角质细胞生长因子1/成纤维细胞生长因子7
|
Synonyms
|
FGF7; FGF-7; FGF-7; fibroblast growth factor 7HBGF-7; Heparin-binding growth factor 7; keratinocyte growth factor; KGFfibroblast growth factor 7 (keratinocyte growth factor)
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10ng/ml, corresponding to a specific activity of >1.0×105IU/mg.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered solution in 20mM PB, 0.5M NaCl, pH8.0.
|
Endotoxin
|
Less than 1EU/μg of rHuKGF-1/FGF-7 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Human KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparin sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51% amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, human KGF-1 shares 96% and 92% amino acid sequence identity with murine and rat FGF-7, respectively.
|
Amino Acid Sequence
|
CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK EQKTAHFLPM AIT
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5466-2μg
|
Recombinant Human KGF-1/FGF-7
|
2μg
|
P5466-10μg
|
Recombinant Human KGF-1/FGF-7
|
10μg
|
P5466-100μg
|
Recombinant Human KGF-1/FGF-7
|
100μg
|
P5466-1mg
|
Recombinant Human KGF-1/FGF-7
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。