Recombinant Human IL-36 Receptor Antagonist

Name

Recombinant Human IL-36 Receptor Antagonist (Recombinant Human Interleukin-36 Receptor Antagonist; rHuIL-36RA)；重组人白细胞介素36受体拮抗剂

Synonyms

IL36RN; FIL1D; IL1F5; IL1HY1; IL1L1; IL1RP3; UNQ1896/PRO4342

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg in the presence of 20ng/ml of recombinant human IL-36 beta.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuIL-36RA as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91%a.a. sequence identity with murine IL-36RA.

Amino Acid Sequence

VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD

产品编号

产品名称

包装

KL-5273-5μg

Recombinant Human IL-36 Receptor Antagonist

5μg

KL-5273-25μg

Recombinant Human IL-36 Receptor Antagonist

25μg

KL-5273-100μg

Recombinant Human IL-36 Receptor Antagonist

100μg

KL-5273-1mg

Recombinant Human IL-36 Receptor Antagonist

1mg

-

说明书

1份