产品编号 |
产品名称 |
产品包装 |
产品价格 |
P5273-5μg |
Recombinant Human IL-36 Receptor Antagonist |
5μg |
464.00元 |
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
Human
|
26525
|
Q9UBH0
|
E. coli
|
154aa
|
16.8kDa
|
-
|
About this protein
|
Name
|
Recombinant Human IL-36 Receptor Antagonist (Recombinant Human Interleukin-36 Receptor Antagonist; rHuIL-36RA);重组人白细胞介素36受体拮抗剂
|
Synonyms
|
IL36RN; FIL1D; IL1F5; IL1HY1; IL1L1; IL1RP3; UNQ1896/PRO4342
|
Purity
|
>95% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-36 beta induced IL-8 secretion by human preadipocytes is less than 1.0μg/ml, corresponding to a specific activity of >1000IU/mg in the presence of 20ng/ml of recombinant human IL-36 beta.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
Endotoxin
|
Less than 1EU/μg of rHuIL-36RA as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
Category
|
Cytokine
|
Background
|
Interleukin-36 receptor antagonist (IL-36RA) is a secreted protein which belongs to the interleukin 1 cytokine family (IL-1 family) and it is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RA has been reported to antagonize the biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Furthermore, it could constitute part of an independent signaling system analogous to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist and interleukin-1 receptor type I (IL-1R1). In addition, The receptor for IL-36RA has not been positively identified. Indirect evidence suggests it is IL-1Rrp2. Recombinant human IL-36 RA contains 154 amino acid residues and it shares 91%a.a. sequence identity with murine IL-36RA.
|
Amino Acid Sequence
|
VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAGKVIKGEE ISVVPNRWLD ASLSPVILGV QGGSQCLSCG VGQEPTLTLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTVPEADQPV RLTQLPENGG WNAPITDFYF QQCD
|
包装清单:
产品编号
|
产品名称
|
包装
|
P5273-5μg
|
Recombinant Human IL-36 Receptor Antagonist
|
5μg
|
P5273-25μg
|
Recombinant Human IL-36 Receptor Antagonist
|
25μg
|
P5273-100μg
|
Recombinant Human IL-36 Receptor Antagonist
|
100μg
|
P5273-1mg
|
Recombinant Human IL-36 Receptor Antagonist
|
1mg
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。