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网站主页化工产品目录生物化工抗体一抗其他一抗 Beclin 1 antibody

Beclin-1 antibody

￥3308 100ug 起订

其他更新日期：2020-01-12

ProSci Incorporated

非会员

联系人：妙通生物

电话：021-64169739-803拨打

手机：18702149899 拨打

邮箱：ypge@mt-bio.com

产品详情:

英文名称：: Beclin-1 antibody

应用范围：: E, WB, IHC

抗体英文名：: ProSci incorporated

适应物种：: H, M, R

宿主：: 兔

保存条件：: -20℃~-80℃

抗原来源：: 多肽

货号：: 3613

标记物：: 不限

抗原：: 多肽

供应商：: 普赛生物（苏州）有限公司

保质期：: 三年

数量：: 中量

规格：: 100ug

是否单克隆：: 单克隆

细胞自噬是真核生物在保守进化中对细胞内物质进行周转的重要过程，该过程中一些损坏的蛋白或细胞器被双层膜结构的自噬小泡包裹后，送入溶酶体中进行降解并得以循环利用。动物细胞衰老反应过程中，往往发生分子伴侣介导的自噬过程，细胞自噬与细胞凋亡、细胞衰老一样，是十分重要的生物学现象，它参与生物的发育、生长等多种过程，细胞自噬的异常易导致癌细胞的出现。ProSci有很多种细胞自噬抗体，其中包括自噬损害调节器DRAM抗体和自噬相关蛋白APG7、PIST、LAMP-1、LAMP-2抗体。此外，ProSci还提供自噬检测试剂盒。ProSci的每种自噬抗体都可适用于ELISA，western blot和免疫组织化学（IHC）。

Beclin-1 Antibody | catalog# 3613

Background

Beclin-1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death.

Description

Western blot analysis of Beclin-1 in 293 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.

Immunohistochemistry of beclin-1 in rat brain tissue with beclin-1 antibody at 2 μg/mL.

Specifications

Additional Names

Beclin-1 Antibody: Beclin-1, Coiled-coil myosin-like Bcl-2-interacting protein

Disclaimer

This product is for research use only.

User Note

Optimal dilutions for each application to be determined by the researcher.

Formulation

Physical State: Liquid

Concentration: 1 mg/mL

Isotype

IgG

Clonality/Clone

This is a polyclonal Beclin-1 Antibody.

Conjugate

None

Purification

Beclin-1 Antibody is affinity chromatography purified via peptide column.

Host

Beclin-1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Beclin-1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1.

Application

Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.

Tested Application

E, WB, IHC

Buffer

Beclin-1 Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3613P - Beclin-1 Peptide

Storage

Beclin-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

Cat. No. 1210 - 293 Cell Lysate
- Cat. No. 1403 - Mouse Brain Tissue Lysate]

Species Reactivity

H, M, R

Protein GI Number

16307457

Protein Accession Number

AAH10276

Reference

Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
  - Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
    - Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.]