产品编号 |
产品名称 |
产品包装 |
产品价格 |
AF2152 |
NQO2 Rabbit Monoclonal Antibody |
50μl |
618.00元 |
来源
|
用途
|
交叉反应性
|
分子量
|
Rabbit
|
WB, FC
|
H, M, R
|
26.0kDa
|
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
1:1,000-1:2,000
|
-
|
-
|
-
|
-
|
1:10-1:50
|
-
|
-
|
抗体详细信息如下:
About this Antibody
|
Name
|
NQO2 Rabbit Monoclonal Antibody
|
Category
|
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
|
Isotype
|
IgG
|
Purification
|
Affinity purification
|
About the Immunogen
|
Immunogen
|
Recombinant protein
|
Gene ID
|
4835(Human); 18105(Mouse); 291084(Rat)
|
SwissProt
|
P16083(Human); Q9JI75(Mouse); Q6AY80(Rat)
|
Synonyms
|
DHQV; DIA6; NMOR2; QR2
|
Category
|
Metabolism
|
Background
|
NAD(P)H:quinone oxidoreductase 1 (NQO1) and NRH:quinone oxidoreductase (NQO2) are flavoproteins that catalyze the metabolic detoxification of quinones and their derivatives to hydroquinones. This detoxification process protects cells against quinone-induced oxidative stress, cytotoxicity and mutagenicity. NQO2 is a 231 amino acid protein and is 43 amino acids shorter than NQO1 at its C-terminus. NQO2 is an isozyme of NQO1 and transfers two electrons to a quinone, resulting in the formation of a hydroquinone product . The NQO2 gene is ubiquitously expressed and induced in response to TCDD. NQO2 has a higher level of expression in mouse liver and testis than NQO1, which is highly expressed in the heart. NQO2 has a different cofactor requirement than NQO1 and uses dihydronicotinamide riboside (NRH) rather than NAD(P)H as an electron donor. Unlike NQO1, NQO2 is resistant to typical inhibitors of NQO1 such as dicoumarol, Cibacron blue and phenindonee, but is inhibited by quercetin and benzo(a)pyrene. NQO2 contains a specific metal binding site, which is absent in NQO1 and several cis-elements including SP1 binding sites, CCAAT box, XRE and ARE, which are located at the NQO2 gene promoter.
|
包装清单:
产品编号
|
产品名称
|
包装
|
AF2152
|
NQO2 Rabbit Monoclonal Antibody
|
50μl
|
AZ050
|
Western一抗稀释液
|
50ml
|
-
|
说明书
|
1份
|
保存条件:
NQO2 Rabbit Monoclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得的检测效果,请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。