293T专用转染试剂

产品介绍

Entranster™-H 是其研发的纳米聚合物转染试剂，针对 HEK293 全系优化，细胞毒性可控，覆盖从微孔板到数十升悬浮放大全场景。

本品适合在 HEK293 全系（HEK293、HEK293T、HEK293T-suspension、HEK293F、HEK293-6E、Expi293 等）细胞中进行贴壁转染、大规模悬浮转染、病毒包装（慢病毒、AAV、腺病毒）与重组蛋白生产。既支持贴壁培养也支持悬浮培养。

可用于大规模正向转染，也可用于高通量筛选的反向转染

可用于病毒包装，也可以用于重组蛋白转染

既支持贴壁培养也支持悬浮培养。

应用本产品文献

1. Cell Death Differ. 2026 Jun 26.doi: 10.1038/s41418-026-01802-w. SUMOylation-stabilized G6PD orchestrates metabolic rewiring for oxidative stress survival and chemoresistance in HCC via a PKCδ-phosphorylation trigger SUMO化稳定的葡萄糖-6-磷酸脱氢酶（G6PD）通过蛋白激酶Cδ（PKCδ）磷酸化触发机制，调控代谢重编程，以促进肝细胞癌（HCC）在氧化应激下的存活和化疗耐药性 (IF:13.6).

2. Dev Comp Immunol. 2026 Jun:179:105625.doi: 10.1016/j.dci.2026.105625. Epub 2026 May 12.  Bombyx mori IML-2 modulates intracellular reactive oxygen species levels and downregulates glycolytic flux to inhibit viral infection  家蚕IML-2调节细胞内活性氧水平并下调糖酵解流量以抑制病毒感染

3. Pestic Biochem Physiol. 2026 May:220:107056.doi: 10.1016/j.pestbp.2026.107056. Epub 2026 Mar 7.

4. Cell Death Dis. 2026 May 16.doi: 10.1038/s41419-026-08872-1.  Targeting lysine-specific demethylase 1 inhibits melanoma metastasis via the NF2-Hippo-YAP pathway  靶向赖氨酸特异性脱甲基酶1可通过NF2-Hippo-YAP通路抑制黑色素瘤转移 (IF:9.6).

5. Nat Commun. 2026 Apr 20.doi: 10.1038/s41467-026-72181-6. Photoactivatable CRISPR/Cas13d via upconversion nanoparticles for deep tissue RNA engineering and orthopedic therapy 通过上转换纳米颗粒实现光激活CRISPR/Cas13d，用于深层组织RNA工程和骨科治疗(IF:15.7).

6. Viruses. 2026 Mar 5;18(3):323.doi: 10.3390/v18030323. Tree Shrew Genome-Wide CRISPR Screen Identifies RNF6 as a Proviral Host Factor for Zika Virus Replication in Brain Microvascular Endothelial Cells 树鼩全基因组CRISPR筛选鉴定出RNF6是寨卡病毒在脑微血管内皮细胞中复制的病毒前体宿主因子

7. Cell Rep Methods. 2026 Feb 11;6(2):101299.doi: 10.1016/j.crmeth.2025.101299. An orthogonal CRISPR/Cpf1 platform for precise spatiotemporal gene regulation and osteoporotic fracture repair. (IF:5.8).

8. J Orthop Surg Res. 2025 Dec 31;21(1):68.doi: 10.1186/s13018-025-06569-3. The functional mechanism and clinical significance of miR-3651 in the healing process of Pilon fractures. (IF:3.8).

9. Aging Cell. 2025 Dec 9;25(1):e70306.doi: 10.1111/acel.70306. FoxO1 Responses to Chronic Oxidative Stress to Participate in Age-Related Osteoporosis by Depriving β-Catenin From TCF7. (IF:7.7).

10. Biochem Pharmacol. 2025 Oct 3;242(Pt 4):117403.doi: 10.1016/j.bcp.2025.117403 Plectin promotes bone formation via phase separation and sequestering annexin A2 Plec通过相分离和螯合Anxa2促进骨形成(IF:5.6).

11. Gene. 2025 Sep 15:965:149650.doi: 10.1016/j.gene.2025.149650. Epub 2025 Jun 30. Contribution of cysteamine dioxygenase to taurine biosynthesis in the oyster Crassostrea gigas半胱胺双加氧酶对牡蛎牛磺酸生物合成的贡献

12. Trends Biotechnol. 2025 Aug 30:S0167-7799(25)00314-2.doi: 10.1016/j.tibtech.2025.07.029. Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase 基于猪逆转录病毒逆转录酶的高效哺乳动物基因组编辑引物 (IF:14.900).