Recombinant Rat KGF-2/FGF-10 (Recombinant Rat Keratinocyte Growth Factor-2/FGF-10; rRtKGF-2/FGF-10);重组大鼠角质细胞生长因子2/成纤维细胞生长因子10
Synonyms
KGF-2; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
Purity
>97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120ng/ml, corresponding to a specific activity of >8.3×103IU/mg.
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris, 500mM NaCl, pH7.4, 5% trehalose.
Endotoxin
Less than 1EU/μg of rRtKGF-2/FGF-10 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
FGF-10 was originally identified from rat embryos by homology-based polymerase chain reaction. Rat FGF-10 shares approximately 95% amino acid sequence identity with human FGF-10. Among the FGF family members, FGF-10 is most closely related to FGF-7. The expression of FGF-10 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant FGF-10 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, FGF-10 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.