Recombinant Human KGF-2/FGF-10

Name

Recombinant Human KGF-2/FGF-10 (Recombinant Human Keratinocyte Growth Factor-2/FGF-10; rHuKGF-2/FGF-10)；重组人角质细胞生长因子2/成纤维细胞生长因子10

Synonyms

KGF-2; FGF-10; fibroblast growth factor 10; Keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5ng/ml, corresponding to a specific activity of >2.0×10⁶IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuKGF-2/FGF-10 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant human FGF-10 contains a 208 amino acids and it shares 92% and 95% amino acid sequence identity with murine and rat FGF-10. Defects in FGF-10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands and lacrimo-auriculo-dento-digital syndrome.

Amino Acid Sequence

LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS

产品编号

产品名称

包装

KL-5475-5μg

Recombinant Human KGF-2/FGF-10

5μg

KL-5475-25μg

Recombinant Human KGF-2/FGF-10

25μg

KL-5475-100μg

Recombinant Human KGF-2/FGF-10

100μg

KL-5475-1mg

Recombinant Human KGF-2/FGF-10

1mg

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说明书

1份