Eotaxin; C-C motif chemokine 11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0ng/ml.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH6.9.
Endotoxin
Less than 0.1EU/μg of rRhEotaxin/CCL11 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Chemokines
Background
CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Recombinant Rhesus Macaque CCL11 is a 74 amino acid (a.a.) protein and it shares 89%, and 57%, and 60%a.a. sequence identity with human, murine and rat CCL11.