高效电转染试剂(核转染)

产品介绍

Entranster^TM-E是英格恩生物公司（Engreen Biosystem Co,Ltd.）专门针对常规方法“难转染”细胞而开发的电穿孔转染试剂。该电转染试剂加入了独有的入核和细胞膜修复成分，使细胞膜更易于被电穿孔，并直接将DNA送入核内，同时降低由于电击造成的细胞死亡率。

应用范围

干细胞，神经细胞，原代细胞和其他方法难转染细胞的转染。

可用于瞬时转染和稳定转染。

主要特点

高转染率Entranster^TM-E含有独有的入核和细胞膜修复成分，可以直接将DNA送入核内，高效转染、快速表达。

低细胞死亡率细胞死亡率<40%。Entranster^TM-E独特的成分能够迅速修复因电击打开的细胞膜，将电击给细胞造成的伤害减到最小。

不需要购置新仪器，常见电转仪都能用 用于Lonza电转仪，单一液体用于多种细胞，效率更好，花费减半。用于Bio-rad、BTX等电转仪，比PBS等效率提高10倍！

应用于本产品的文献

1. STAR Protoc. 2026 Jul 7;7(3):104691.doi: 10.1016/j.xpro.2026.104691. Protocol for generating multiplexed prime-edited monoclonal cell lines in porcine fetal fibroblasts 猪胎儿成纤维细胞中生成多路复用、经过主编辑的单克隆细胞系的方案

2. BMC Biotechnol. 2026 May 9;26(1):83.doi: 10.1186/s12896-026-01164-8. PAM-flexible SpCas9 variants expand the targeting scope for porcine genome editing and cellular disease modeling PAM灵活型SpCas9变体扩大了猪基因组编辑和细胞疾病建模的靶向范围 (IF:4.8).

3. Vet Sci. 2026 May 6;13(5):455.doi: 10.3390/vetsci13050455. Efficient and Safe Knockout of AR and DMRT1 Mediated by Cytosine Base Editors in Chicken DF-1 and PGCs胞嘧啶碱基编辑器在鸡DF-1和原始生殖细胞中高效且安全地敲除AR和DMRT1

4. Trends Biotechnol. 2025 Aug 30:S0167-7799(25)00314-2.doi: 10.1016/j.tibtech.2025.07.029. Highly efficient prime editors for mammalian genome editing based on porcine retrovirus reverse transcriptase 基于猪逆转录病毒逆转录酶的高效哺乳动物基因组编辑引物 (IF:14.900).

5. Nat Biotechnol. 2025 Apr 21;44(3):387-393.doi: 10.1038/s41587-025-02641-9. QBEmax is a sequence-permuted and internally protected base editor. (IF:44.5).

6. Animals (Basel). 2025 Feb 18;15(4):590.doi: 10.3390/ani15040590. Achieving Optimal Transfection Conditions in Chicken Primordial Germ Cells Under Feeder- and Serum-Free Medium 在饲养和无血清培养基下实现鸡原始生殖细胞的最佳转染条件

7. Sci Rep. 2024 Jul 26;14(1):17665.doi: 10.1038/s41598-024-68169-1. Sodium Tanshinone IIA Sulfonate alleviates vascular senescence in diabetic mice by modulating the A20-NFκB-NLRP3 inflammasome-catalase pathway 通过调节A20-NFκB-NLRP3炎性体过氧化氢酶途径缓解糖尿病小鼠血管衰老 (IF:4.122).

8. Biol Direct. 2024 Jun 24;19(1):49.doi: 10.1186/s13062-024-00490-1. Silencing LINC00987 ameliorates adriamycin resistance of acute myeloid leukemia via miR-4458/HMGA2 axis 通过miR-4458/HMGA2轴改善急性髓系白血病的阿霉素耐药性 (IF:5.700).

9. Acta Biochim Biophys Sin (Shanghai). 2024 Jun 25;56(6):892-904.doi: 10.3724/abbs.2024032. FGF21 overexpression alleviates VSMC senescence in diabetic mice by modulating the SYK-NLRP3 inflammasome-PPARγ-catalase pathway FGF21过表达通过调节SYK-NLRP3炎性体PPARγ-过氧化氢酶途径缓解糖尿病小鼠VSMC衰老

10. Parasite  . 2023:30:55. doi: 10.1051/parasite/2023060. Epub 2023 Dec 11. Comparative characterization of microRNA-71 of Echinococcus granulosus exosomes

11. BMC Biotechnol. 2023 Aug 16;23(1):29.doi: 10.1186/s12896-023-00799-1. Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system 使用Lonza 4D Nucleofector系统在猪胎儿成纤维细胞中高效递送大尺寸Cas9-EGFP载体

12. Vet Sci. 2023 Jan 19;10(2):75.doi: 10.3390/vetsci10020075. Transcriptome RNA Sequencing Reveals That Circular RNAs Are Abundantly Expressed in Embryonic Breast Muscle of Duck  circRNA在鸭胚胎胸肌中大量表达

13. Genes (Basel). 2022 May 7;13(5):835.doi: 10.3390/genes13050835. (IF4.096)   Efficient Simultaneous Introduction of Premature Stop Codons in Three Tumor Suppressor Genes in PFFs via a Cytosine Base Editor 通过胞嘧啶碱基编辑器在PFFs的三个肿瘤抑制基因中同时有效引入过早终止密码子

14. iScience. 2022 Apr 13;25(5):104249.doi: 10.1016/j.isci.2022.104249. L3MBTL2-mediated CGA transcriptional suppression promotes pancreatic cancer progression through modulating autophagy. (IF:4.5).

15. Oncol Lett.2021 Jul;22(1):521.doi: 10.3892/ol.2021.12782. Epub 2021 May 11. GFI-1 overexpression promotes cell proliferation and apoptosis resistance in mycosis fungoides by repressing Bax and P21

16. Veterinary MicrobiologyVolume 239, December 2019, 108494
    Bovine herpesvirus 1 tegument protein UL41 suppresses antiviral innate immune response via directly targeting STAT1牛疱疹病毒1型被膜蛋白UL41抑制抗病毒天然免疫

17. J Cell Biochem . 2018 Dec;119(12):10176-10185. doi: 10.1002/jcb.27358. Epub 2018 Aug 20. Excreted-secreted antigens of Toxoplasma gondii inhibit Foxp3 via IL-2Rγ/JAK3/Stats pathway

18. J Cell Mol Med. 2017 Mar 16;21(9):1944-1953.doi: 10.1111/jcmm.13115. Chinese 1 strain of Toxoplasma gondii excreted-secreted antigens negatively modulate Foxp3 via inhibition of the TGFßRII/Smad2/Smad3/Smad4 pathway. (IF:4.7).