抗体 Anti-AMPK b1 Antibody (8X437)|TMAB-02529|TargetMol

中文名称：

抗体 Anti-AMPK beta 1 Monoclonal Antibody (8X437)

英文名称：

Anti-AMPK beta 1 Monoclonal Antibody (8X437)

品牌：

TargetMol

产地：

美国

保存条件：

Store at low temperature -20°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

产品类别：

抑制剂

重组：

Hybridoma Monoclonal Antibody

种属：

Human

应用：

WB,IHC-F,IHC-P,ICC/IF,IP

反应性：

Human,Mouse,Rat,Monkey

宿主：

Mouse

偶联物：

Unconjugated

靶点：

PRKAB1

克隆性：

Monoclonal

免疫原：

Purified recombinant human AMPK beta 1 protein fragments expressed in E. coli

亚型：

IgG2a

验证方法：

1. Sample: Cerebrum (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg U87MG (Human) Cell Lysate at 30 μg U251 (Human) Cell Lysate at 30 μg Primary: Anti-AMPK b1 (TMAB-02529) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 38 kD 2. Sample: Cerebrum (Rat) Lysate at 40 μg Primary: Anti-AMPK b1 (TMAB-02529) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 38 kD 3. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK b1) Monoclonal Antibody, Unconjugated (TMAB-02529) at 1:800 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

UniProt ID：

Q9Y478

克隆号：

8X437

浓度：

1mg/ml

基因名称：

PRKAB1

货号：

T014|TMAB-02529