| 名称 | Physostigmine |
| 描述 | Physostigmine is an alkaloid extracted from the poison bean (Physostigma venenosum) and acts as a reversible acetylcholinesterase (AChE) inhibitor. Physostigmine inhibits the action of acetylcholinesterase, increasing acetylcholine levels, and thus serves as an antidote for anticholinergic poisoning. Physostigmine crosses the blood-brain barrier and stimulates central cholinergic neurotransmission. Physostigmine can reverse memory deficits in transgenic mice with Alzheimer's disease. |
| 体外活性 | 方法：取海马CA3-CA1区组织匀浆，经10 μM Physostigmine灌流20 min后，分别于立即、洗脱20 min和洗脱1 h时，采用比色法检测乙酰胆碱酯酶(AChE)活性。
结果：Physostigmine 灌流20 min时AChE活性上升，洗脱20 min后恢复，洗脱1 h后出现代偿性升高。[1]
方法：原代大鼠皮层神经元加入Physostigmine (0.01, 0.1, 1.0, 10, 100 nM) 处理24 h，活细胞成像(每2秒1帧，共3分钟)，生成kymograph，KymoAnalyzer分析。
结果：各浓度Physostigmine对轴突运输速度(顺行)均无显著影响；最高浓度(100 nM)反而使逆行速度显著增加。[2] |
| 体内活性 | 方法：为研究Physostigmine的作用，成年C57BL/6J雄性小鼠腹腔注射Physostigmine(0.25 mg/kg)，注射后20 min腹腔注射氯胺酮(5 mg/kg)，50 min后测 强迫游泳实验，记录后4 min内不动时间。
结果：Physostigmine显著提高不动时间；氯胺酮显著降低Physostigmine诱导的不动时间增加,表明Physostigmine损害神经传递。[1] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 40 mg/mL (145.27 mM), Sonication is recommended.
H2O : Insoluble
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| 关键字 | Physostol | Physostigmine | Ezerin | Esromiotin | Antilirium |
| 相关产品 | 4-Methylbenzylidene camphor | Methyl tridecanoate | Trimethylammonium chloride | Ethyl (triphenylphosphoranylidene) acetate | Diazinon | Coumaran | Diethyltoluamide | Pralidoxime Iodide | Dimethyl sulfoxide | Uridine 5'-monophosphate disodium salt | Methylene Violet 3RAX | Propoxur |
