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p27 KIP 1 抗体

品牌：MedChemExpress (MCE)

货号：HY-P85705

存储条件：-20°C保存1年。避免重复冻结/解冻循环。

运输条件：用蓝冰运输。

产品活性：p27 KIP 1 Antibody (YA5397) 是一种小鼠来源、无偶联标记、抗 P27 Kip1 的单克隆抗体。它可用于人、小鼠、大鼠背景下的 IHC-P、WB 实验。

研究背景：P27 Kip1 是一种多功能蛋白，在氮代谢中扮演酶、转录共调节因子和分子伴侣的角色。它催化 ATP 依赖的谷氨酰胺合成反应，将谷氨酸 (glutamate) 和氨 (ammonia) 转化为谷氨酰胺 (glutamine)。反馈抑制状态的 GlnA 能与转录调节因子 TnrA 相互作用并调控其活性：在氮限制条件下，TnrA 保持 DNA 结合活性状态并激活氮同化相关基因的转录；而在氮过量条件下，反馈抑制的 GlnA 会与 TnrA 形成稳定复合物，抑制其 DNA 结合活性。此外，反馈抑制的 GlnA 还能作为分子伴侣稳定 GlnR 的 DNA 结合活性，从而抑制氮同化基因的转录。

宿主：Mouse

反应物种：Human, Mouse, Rat

推荐稀释比例：IHC1:50-200; WB: 1:1000-2000 Immunohistochemical analysis of paraffin-embedded mouse colon tissue using P27 Kip1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using P27 Kip1 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

基因 ID：1027

克隆性：Monoclonal

偶联：Non-conjugated

修饰：Unmodified

蛋白数据库：P46527

详情参考：www.medchemexpress.cn/antibody/p27-kip1-antibody-ya5397.html