Biotinylated人Her2 / ErbB2蛋白, His Tag, ultra sensitivity

Biotinylated Human Her2 / ErbB2 Protein, His Tag, ultra sensitivity (primary amine labeling, long spacer) (MALS verified)

分子别名（Synonym）

ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1

表达区间及表达系统（Source）

MABSol® Biotinylated Human Her2, His Tag, primary amine labeling, long spacer (HE2-H822R) is expressed from human HEK293 cells. It contains AA Thr 23 - Thr 652 (Accession # P04626-1). It is the biotinylated form of Human Her2, His Tag (Cat. No. HE2-H5225).

Predicted N-terminus: Thr 23

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 70.2 kDa. The protein migrates as 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A specially optimized long-spacer biotin reagent (32.5 angstroms) is used in this product to minimize potential steric hindrance.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

人表皮生长因子受体2（HER2）又称ERBB2、HER-2、HER-2/neu、NEU、NGL、TKR1及c-erb B2，是一种导致乳腺癌侵袭性更强的蛋白质。作为ErbB蛋白家族（通常称为表皮生长因子受体家族）的成员，HER2是一种细胞膜表面结合的受体酪氨酸激酶，通常参与调控细胞生长和分化的信号转导通路。

HER2被认为是一种孤儿受体，表皮生长因子家族的配体均无法将其激活。约30%的乳腺癌存在HER2基因扩增或其蛋白产物的过度表达。该受体在乳腺癌中的过度表达与疾病复发率升高及预后不良相关。HER2在发育、癌症发生、神经肌肉接头信号传递以及细胞生长与分化调控过程中发挥重要作用。