人BAFF蛋白, His, Avitag

Biotinylated Human BAFF / TNFSF13B / CD257 Protein, His,Avitag™, active trimer (MALS verified)

分子别名（Synonym）

TNFSF13B,BAFF,BLYS,CD257,DTL,TALL1,THANK,TNFSF20,ZTNF4,TALL-1

表达区间及表达系统（Source）

Biotinylated Human BAFF Protein, His,Avitag (BAF-H82Q2) is expressed from human 293 cells (HEK293). It contains AA Ala 134 - Leu 285 (Accession # AAH20674.1).

Predicted N-terminus: His

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 20.2 kDa. The protein migrates as 21-23 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

B细胞活化因子（BAFF）亦称肿瘤坏死因子配体超家族成员13B（TNFSF13B）、B淋巴细胞刺激因子（BLyS）、分化簇257（CD257）、DTL、TNF及APOL相关白细胞表达配体（TALL-1）、THANK、TNFSF20、ZTNF4，属于肿瘤坏死因子（TNF）配体家族细胞因子。该细胞因子是受体TNFRSF13B/TACI、TNFRSF17/BCMA和TNFRSF13C/BAFFR的配体，表达于B细胞谱系中，可作为有效的B细胞活化剂。研究表明，其在B细胞增殖与分化过程中起关键作用。

BAFF以跨膜蛋白形式表达于单核细胞、树突状细胞及骨髓基质细胞等多种细胞表面。作为三种特殊肿瘤坏死因子受体（BAFF-R、TACI和BCMA）的天然配体，BAFF与这些受体的结合亲和力各不相同：TACI因对BAFF类似蛋白——增殖诱导配体（APRIL）具有更高亲和力，故结合效果最弱；BCMA则呈现中等结合特性，可不同程度地与BAFF或APRIL协同作用。BAFF-R和BCMA的信号传导能刺激B淋巴细胞增殖并抑制细胞凋亡。

尽管已知BAFF以异源或同源三聚体形式发挥活性，但这些配体均以异源三聚体形式（即三个相同分子组合）与异源三聚体受体相互作用。作为高效的B细胞活化剂，BAFF在B细胞增殖与分化过程中具有重要调控功能。