人CTLA-4 / CD152蛋白, His Tag

Human CTLA-4 / CD152 Protein, His Tag

分子别名（Synonym）

CTLA4, CD152

表达区间及表达系统（Source）

Human CTLA-4, His Tag (CT4-H5229) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).

Predicted N-terminus: Ala 37

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 15.4 kDa. The protein migrates as 20-25 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 6 months under sterile conditions after reconstitution.

背景介绍

细胞毒性T淋巴细胞抗原4（CTLA-4）亦称分化簇152（CD152），是一种可下调免疫系统活性的蛋白质受体。该分子属于免疫球蛋白超家族成员，表达于辅助性T细胞表面，可向T细胞传递抑制性信号。其蛋白质结构包含胞外V结构域、跨膜结构域及胞质尾部，存在编码不同异构体的选择性剪接变体。

CTLA4与T细胞共刺激蛋白CD28具有相似性，两者均可结合抗原呈递细胞表面的CD80（B7-1）和CD86（B7-2）分子。不同的是，CTLA4传递抑制性信号，而CD28传递刺激性信号。在调节性T细胞中亦发现细胞内CTLA4的存在，该分子可能对其功能至关重要。目前CTLA4与抗体融合蛋白（CTLA4-Ig）已应用于类风湿关节炎的临床试验。