人EGF蛋白, His Tag

Human EGF Protein, His Tag

分子别名（Synonym）

EGF, URG, HOMG4

表达区间及表达系统（Source）

Human EGF Protein, His Tag (EGF-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-11 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method / rFC method.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景介绍

人表皮生长因子（EGF）亦称HOMG4或URG，是一种通过与其受体EGFR结合，在细胞生长、增殖和分化调节中发挥重要作用的生长因子。该因子存在于人类血小板、巨噬细胞、尿液、唾液、乳汁及血浆中。EGF是EGF蛋白家族的创始成员，该家族各成员具有高度相似的结构与功能特征——所有成员均包含一个或多个保守氨基酸序列重复单元。

唾液EGF的生物学效应包括：促进口腔及胃食管溃疡愈合、抑制胃酸分泌、刺激维生素D合成，以及对黏膜的保护作用（抵御胃酸、胆汁酸、胰蛋白酶、胰凝乳蛋白酶等管腔内损伤因子，以及物理、化学和细菌性侵害）。由于EGF会增加癌症风险，抑制其活性可降低患癌风险。