人Her2 / ErbB2蛋白, His, Avitag, premium grade

Biotinylated Human Her2 / ErbB2 Protein, His,Avitag™, premium grade

分子别名（Synonym）

ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1

表达区间及表达系统（Source）

Biotinylated Human Her2, His,Avitag, premium grade (HE2-H82E2) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 652 (Accession # P04626-1).

Predicted N-terminus: Thr 23

该产品在严格的质量控制体系下生产，执行包括无菌检测和内毒素检测在内的全方位测试。产品性能经过严谨验证，并针对细胞培养用途或早期临床前阶段的其他应用进行兼容性测试。当您准备进入后期临床阶段时，我们还可提供定制GMP蛋白服务：根据您的具体需求，我们将协同开发符合GMP级别的产品，同时满足基于细胞治疗（如CAR-T等）的细胞制造过程中原辅材料使用规范。

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 72.9 kDa. The protein migrates as 101 kDa±3 kDa under reducing (R) condition, and 94 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

标记（Labeling）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotinylation）

As determined by Quantitative ELISA binding assay against streptavidin.

内毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method / rFC method.

宿主蛋白残留（Host Cell Protein）

<0.5 ng/µg of protein tested by ELISA.

宿主核酸残留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

无菌（Sterility）

Negative

支原体（Mycoplasma）

Negative.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 24 months in lyophilized state;

-70°C for 12 months under sterile conditions after reconstitution.

背景介绍

人表皮生长因子受体2（HER2），亦称ERBB2、HER-2、HER-2/neu、NEU、NGL、TKR1及c-erb B2，是一种导致乳腺癌侵袭性增强的关键蛋白。作为ErbB蛋白家族（通常称为表皮生长因子受体家族）成员，HER2是一种细胞膜表面结合的受体酪氨酸激酶，正常参与调控细胞生长与分化的信号转导通路。

HER2被认为是孤儿受体（注：其配体尚未明确），表皮生长因子（EGF）家族配体均无法将其激活。约30%的乳腺癌存在HER2基因扩增或其蛋白产物的过表达，这种过表达与疾病复发率升高及预后较差显著相关。研究显示，HER2在个体发育、肿瘤发生、神经肌肉接头信号传递以及细胞生长分化调控中均发挥重要作用。