Phospho-4E BP1 (Thr37)抗体;Phospho-4E BP1 (Thr37) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-4E BP1 (Thr37) in MDAMB435 lysates using Phospho-4E BP1 (Thr37) antibody.    

  
  

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     Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using 4EBP1 (Phospho-Thr36) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.    

  
  

The Phospho-4E-BP1 (Thr37) antibody is a critical tool for studying the regulation of protein synthesis and cellular growth through the mTOR (mammalian target of rapamycin) signaling pathway. 4E-BP1 (eukaryotic translation initiation factor 4E-binding protein 1) is a downstream effector of mTOR complex 1 (mTORC1). When unphosphorylated, 4E-BP1 binds to eIF4E, a cap-binding protein essential for initiating mRNA translation, thereby inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 at specific residues, including Thr37. Thr46. Ser65. and Thr70. disrupts its interaction with eIF4E, enabling translation machinery assembly. The Thr37 site is among the earliest phosphorylation events in this hierarchical process, mediated by mTORC1 activity.

This antibody specifically detects 4E-BP1 phosphorylated at Thr37. serving as a marker for mTORC1 activation. Researchers use it to investigate cellular responses to growth factors, nutrient availability, stress, or pharmacological inhibitors (e.g., rapamycin) in contexts like cancer, metabolic disorders, and aging. It is widely applied in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to assess signaling dynamics in cell lines, tissues, or preclinical models. Dysregulation of 4E-BP1 phosphorylation is linked to diseases characterized by uncontrolled proliferation, making this antibody valuable for both mechanistic studies and drug development targeting mTOR pathways.