Phospho-4E-BP1 (Ser65)抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using Phospho-4E-BP1 (Ser65) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Jurkat cells using Phospho-4E-BP1 (Ser65) mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using Phospho-4E-BP1 (Ser65) mouse mAb with DAB staining.    

  
  

The Phospho-4E-BP1 (Ser65) antibody detects the phosphorylated form of the eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1) at serine 65. a key regulatory site in cellular translation control. 4E-BP1 inhibits cap-dependent translation by binding to the eukaryotic initiation factor 4E (eIF4E), preventing its interaction with eIF4G to form the eIF4F complex. Phosphorylation of 4E-BP1. particularly at Ser65. disrupts this interaction, releasing eIF4E to promote ribosome recruitment and protein synthesis. This phosphorylation event is primarily mediated by the mechanistic target of rapamycin complex 1 (mTORC1) in response to growth factors, nutrients, or cellular stress, linking it to pathways regulating cell growth, proliferation, and survival.

The Phospho-4E-BP1 (Ser65) antibody is widely used in research to assess mTORC1 activity and downstream signaling in conditions such as cancer, metabolic disorders, and aging. It helps evaluate therapeutic responses to mTOR inhibitors (e.g., rapamycin) or metabolic interventions. Specificity for the phosphorylated Ser65 epitope ensures accurate detection of the active, mTOR-regulated form of 4E-BP1. Applications include Western blotting, immunofluorescence, and immunohistochemistry, often paired with total 4E-BP1 antibodies for normalization. Proper sample preparation (e.g., phosphatase inhibitors) and timing are critical, as phosphorylation is dynamic and context-dependent. This antibody remains a vital tool for studying translational regulation and mTOR pathway dysregulation in disease.