Phospho-Glycogen Synthase (Ser641)抗体;Phospho-Glycogen Synthase (Ser641) Antibody—艾普蒂 新品

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     Western blot analysis of Phospho-Glycogen synthase 1 (S641) in HeLa lysates using Phospho-Glycogen Synthase (Ser641) antibody.    

  
  

Phospho-Glycogen Synthase (Ser641) antibodies are essential tools for studying the regulation of glycogen synthase (GS), a key enzyme in glycogen synthesis. Glycogen synthase catalyzes the addition of glucose molecules to glycogen, a critical energy reservoir in cells. Its activity is tightly controlled by phosphorylation at multiple sites, which inhibits enzyme function. Phosphorylation at serine 641 (Ser641) is a critical regulatory event, primarily mediated by glycogen synthase kinase-3 (GSK-3). This modification is part of a hierarchical phosphorylation mechanism, where prior priming phosphorylation at downstream sites (e.g., Ser645. Ser649. Ser653) facilitates GSK-3-mediated phosphorylation at Ser641.

Insulin signaling promotes dephosphorylation of these sites via the PI3K/Akt pathway, which inactivates GSK-3. thereby activating glycogen synthase to enhance glycogen storage. Conversely, catabolic signals (e.g., glucagon, stress) increase Ser641 phosphorylation, suppressing glycogen synthesis.

Antibodies targeting phospho-Glycogen Synthase (Ser641) enable researchers to detect and quantify this post-translational modification using techniques like Western blotting, immunofluorescence, or ELISA. These antibodies are widely used in metabolic studies, particularly in diabetes, insulin resistance, and glycogen storage disorders, to assess GS regulation. They also help elucidate crosstalk between signaling pathways, such as insulin, AMPK, or mTOR, in energy homeostasis. Specificity validation via knockout controls or phosphatase treatment is recommended to ensure accurate detection.