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网站主页 DiMethyl-Histone H3 (Lys27)抗体 DiMethyl-Histone H3 (Lys27)抗体
  • DiMethyl-Histone H3 (Lys27)抗体;DiMethyl-Histone H3 (Lys27) Antibody—艾普蒂
  • DiMethyl-Histone H3 (Lys27)抗体;DiMethyl-Histone H3 (Lys27) Antibody—艾普蒂
  • DiMethyl-Histone H3 (Lys27)抗体;DiMethyl-Histone H3 (Lys27) Antibody—艾普蒂

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DiMethyl-Histone H3 (Lys27)抗体;DiMethyl-Histone H3 (Lys27) Antibody—艾普蒂 新品

Mouse Monoclonal DiMethyl-HistoneH3(Lys27) Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-06-03

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：nuodina88@163.com

产品详情:

中文名称：
DiMethyl-Histone H3 (Lys27)抗体
英文名称：
Mouse Monoclonal DiMethyl-HistoneH3(Lys27) Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 8914 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
DiMethyl-Histone H3 (Lys27)

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF1/20 Human,Mouse,Rat
IHC咨询技术 Human,Mouse,Rat
ICC1/50-1/200 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa咨询技术 Human,Mouse,Rat
   

产品详情

AliasesH3K27me2; H3 histone; HIST1H3A; Histone cluster 1; H3a
Entrez GeneID8350
clone5H6
WB Predicted band sizeCalculated MW: 15 kDa; Observed MW: 15 kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenSynthetic Peptide of Histone H3 (Di Methyl Lys27)
FormulationPurified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol.

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       Western blot analysis of DiMethyl-Histone H3 (Lys27) in Hela, rat heart, mouse heart, HUVEC, Jurkat lysates using DiMethyl-Histone H3 (Lys27) antibody.    


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       Western blot analysis of DiMethyl-Histone H3 (Lys27) in C6, 3T3, C2C12 lysates using DiMethyl-Histone H3 (Lys27) antibody    


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       Immunoprecipitation analysis of DiMethyl-Histone H3 (Lys27) in Hela lysates using DiMethyl-Histone H3 (Lys27) antibody.    


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       Immunocytochemistry analysis ofDiMethyl-Histone H3 (Lys27) in HeLa cells using DiMethyl-Histone H3 (Lys27) antibody.    


           

参考文献

以下是3篇关于H3K27me2(Dimethyl-Histone H3 Lys27)抗体的经典文献摘要，供参考：

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1. **文献名称**: *Histone H3K27 trimethylation and H3K4 methylation define different chromatin states in Polycomb silencing*

**作者**: Boyer LA et al.

**摘要**: 该研究通过ChIP-seq技术，使用H3K27me2/me3抗体揭示Polycomb复合物(PRC)介导的基因抑制机制，发现H3K27二甲基化与三甲基化在胚胎干细胞分化中具有不同的表观遗传调控功能。

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2. **文献名称**: *Role of histone H3 lysine 27 methylation in Polycomb-group silencing*

**作者**: Cao R et al.

**摘要**: 本文利用H3K27me2特异性抗体进行染色质免疫沉淀实验，证明EZH2(PRC2核心催化亚基)介导的H3K27二甲基化和三甲基化是维持干细胞多能性和基因沉默的关键表观遗传标记。

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3. **文献名称**: *A histone H3 lysine 27-specific antibody validates Drosophila Polycomb function*

**作者**: Pengelly AR et al.

**摘要**: 作者开发并验证了H3K27me2抗体的特异性，通过果蝇模型证明H3K27二甲基化在发育基因调控中与Polycomb抑制复合物协同作用，且其分布模式与三甲基化存在差异。

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4. **文献名称**: *Histone modifications: from genome-wide maps to functional insights*

**作者**: Bernstein BE et al.

**摘要**: 该综述系统总结了H3K27me2等组蛋白修饰的检测方法(如抗体特异性验证)，并探讨其在染色质结构动态调控及癌症等疾病中的潜在作用机制。

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提示：具体实验引用时建议通过PubMed或Google Scholar核对最新文献，关注抗体验证方法(如Western blot/ChIP的示例数据)以确保可靠性。

       

背景信息

The DiMethyl-Histone H3 (Lys27) antibody specifically detects histone H3 proteins dimethylated at lysine 27 (H3K27me2), a post-translational modification critical for epigenetic regulation. Histone H3 is a core component of nucleosomes, and lysine methylation modulates chromatin structure and gene expression. H3K27me2 is associated with transcriptional repression and serves as a dynamic marker of chromatin states. Unlike the well-characterized H3K27me3 (trimethylation), which is strongly linked to Polycomb Repressive Complex 2 (PRC2)-mediated gene silencing in development and cancer, H3K27me2 is more broadly distributed and may play roles in maintaining facultative heterochromatin or priming regions for PRC2 activity.

This antibody is widely used in chromatin immunoprecipitation (ChIP), immunofluorescence (IF), and Western blotting to study epigenetic mechanisms in cellular differentiation, oncogenesis, and stem cell biology. Aberrant H3K27 methylation is implicated in diseases, including malignancies like gliomas and lymphomas, where altered PRC2 activity disrupts normal methylation patterns. Researchers rely on the specificity of H3K27me2 antibodies to distinguish between methylation states, often validating results with demethylase treatments or knockouts. Cross-reactivity with H3K27me1/me3 must be ruled out to ensure accurate interpretation. Understanding H3K27me2 dynamics provides insights into how cells balance gene activation and repression during development and disease progression.

       
DiMethyl-Histone H3 ;DiMethyl-Histone H3 ;DiMethyl-Histone H3 ;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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