HISTONE H3抗体(兔多抗)

背景资料

The nucleosome is made up of four core histone proteins （H2A， H2B， H3 and H4） and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation， phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.

应用类型

Western blotting （0.5-4 ug/ml）and Immunohistochemistry （20 ug/ml）. However， the optimal conditions should be determined individually. The antibody detects 17-20 kDa histone H3 protein. It does not cross-react with other histones.

免疫原

Synthetic peptide near the C-terminus of human Histone H3

来源宿主

兔

反应性

经检测验证，可与人、小鼠、大鼠种属来源的Histone H3蛋白发生免疫反应.

保存建议

提供的Histone H3抗体产品为100 ug 无色溶液，其浓度为0.5 mg/ml，溶解于包含有30%甘油， 0.5% BSA， 0.01%硫柳汞的PBS （pH 7.2）缓冲液中.请将该Histone H3抗体产品置于-20 ℃冷冻保存，建议分装使用以避免反复冻融.