RAP1A+RAP1B抗体;RAP1A+RAP1B Antibody—艾普蒂 新品

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     Western blot analysis of RAP1A + RAP1B expression in Hela cell lysate.    

  
  

RAP1A and RAP1B are closely related small GTPases belonging to the Ras family, sharing ~95% amino acid sequence identity. These proteins function as molecular switches, cycling between active GTP-bound and inactive GDP-bound states to regulate diverse cellular processes, including cell adhesion, migration, proliferation, and differentiation. They play critical roles in integrin-mediated signaling, MAPK/PI3K pathways, and vesicle trafficking. While RAP1A and RAP1B exhibit functional redundancy, studies suggest distinct roles in specific contexts, such as RAP1B's prominence in platelet activation and vascular development.

Antibodies targeting RAP1A+RAP1B are widely used to detect both isoforms simultaneously, given their high homology. These antibodies are valuable tools for studying their combined expression patterns, subcellular localization, and activation status in various physiological and pathological conditions. Validation typically involves knockout cell lines or siRNA-mediated knockdown to confirm specificity. Applications include Western blotting, immunofluorescence, and immunohistochemistry across cancer research (e.g., tumor invasion/metastasis), cardiovascular studies (e.g., angiogenesis), and immunological investigations (e.g., leukocyte adhesion). Researchers must consider potential cross-reactivity with other Ras family members and verify isoform-specific effects through complementary approaches. Commercial antibodies often target conserved regions, with some distinguishing between phosphorylated or GTP-bound active forms.