A-RAF抗体;A-RAF Antibody—艾普蒂

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     Western blot analysis of A-RAF expression in A375 cell lysate.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

A-RAF (ARAF), a member of the RAF kinase family, plays a critical role in the MAPK/ERK signaling pathway, which regulates cell proliferation, differentiation, and survival. Alongside B-RAF and C-RAF, A-RAF activates MEK/ERK through sequential phosphorylation, though its precise biological functions remain less defined compared to its isoforms. A-RAF exhibits tissue-specific expression and distinct regulatory mechanisms, suggesting unique roles in certain cellular contexts. Studies indicate its involvement in developmental processes and potential links to diseases, including cancer, though its oncogenic activity appears weaker than B-RAF's.

A-RAF antibodies are essential tools for detecting and analyzing the protein's expression, localization, and activation states in research. These antibodies enable techniques like Western blotting, immunohistochemistry, and immunofluorescence, helping to elucidate A-RAF's interactions within signaling networks. Due to high sequence homology among RAF isoforms, antibody specificity is crucial to avoid cross-reactivity. Research utilizing A-RAF antibodies has contributed to understanding its role in RAS-driven cancers and resistance mechanisms to targeted therapies. Recent interest focuses on its regulatory phosphorylation sites and potential therapeutic implications in diseases marked by dysregulated MAPK signaling.