ARAF抗体—艾普蒂

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     Lane 1: 15 µg of K-562 lysateLane 2: 15 µg of HeLa lysateLane 3: 15 µg of HEK-293 lysate Result: P16262 can detect ARAF by Western blotting.    

  
  

ARAF (A-Raf proto-oncogene) is a member of the RAF kinase family, which plays a critical role in the MAPK/ERK signaling pathway. This pathway regulates essential cellular processes, including proliferation, differentiation, and survival. ARAF, along with BRAF and CRAF (RAF1), functions as a serine/threonine kinase activated by RAS proteins. Upon activation, RAF kinases phosphorylate MEK1/2. triggering a cascade that ultimately modulates gene expression. While BRAF is the most studied due to its frequent oncogenic mutations in cancers like melanoma, ARAF is less characterized but shares structural similarities with its homologs.

ARAF antibodies are essential tools for studying its expression, localization, and activation in both physiological and pathological contexts. Researchers use these antibodies in techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore ARAF's role in diseases, particularly cancers. Although ARAF mutations are rare compared to BRAF, dysregulated ARAF signaling has been implicated in tumorigenesis, drug resistance, and developmental disorders. For example, certain ARAF variants are linked to Noonan syndrome and other RASopathies.

Despite its lower kinase activity relative to BRAF/CRAF, ARAF's unique interactions with regulatory proteins and context-dependent functions make it a subject of ongoing research. ARAF antibodies also aid in evaluating therapeutic strategies targeting the MAPK pathway, such as RAF inhibitors. However, cross-reactivity with other RAF isoforms remains a technical challenge, necessitating antibody validation. Overall, ARAF antibodies contribute to unraveling the complexity of RAF signaling and its implications in health and disease.