PKM2抗体;PKM2 Antibody—艾普蒂

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     Western blot analysis of PKM2 expression in HeLa cell lysate.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervix cancer, using PKM2 Antibody.    

  
  

PKM2 (pyruvate kinase M2) is a key isoform of the pyruvate kinase enzyme family, which catalyzes the final step of glycolysis by converting phosphoenolpyruvate to pyruvate while generating ATP. Unlike other isoforms, PKM2 is preferentially expressed in rapidly proliferating cells, including cancer cells, where it plays a dual role in regulating metabolic reprogramming and gene transcription. Its unique ability to switch between a highly active tetrameric form (promoting oxidative metabolism) and a less active dimeric form (redirecting glycolytic intermediates to biosynthetic pathways) underpins the Warburg effect, a hallmark of cancer metabolism. PKM2 is also involved in non-metabolic functions, such as acting as a protein kinase or transcriptional coactivator, influencing cell cycle progression, apoptosis, and tumorigenesis.

PKM2 antibodies are essential tools for studying its expression, localization, and post-translational modifications in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PKM2's role in cancer progression, metabolic disorders, and therapeutic targeting. Elevated PKM2 levels are correlated with poor prognosis in various cancers, making it a potential biomarker and therapeutic target. Researchers also utilize these antibodies to explore PKM2's interaction with oncogenic signaling pathways (e.g., HIF-1α, mTOR) and its regulation by metabolites or phosphorylation. Developing specific PKM2 antibodies remains critical for dissecting its complex roles in health and disease.