基本信息
产品名称：PKM2 Antibody
规格或纯度：无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 敲低验证, 见COA
应用：WB, IF/ICC, IHC
特异性：PKM
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：Recombinant PKM2 expressed in E.coli (AA 141-248).
阳性对照：WB: K562, MCF7, HeLa, Hep G2, Raji, Ramos, Jurkat, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer, skin cancer, prostate, prostate cancer, colorectal cancer, liver cancer, Mouse lung, testis, kidney, oviduct tissues. IF/ICC: MCF7, HeLa cells.
偶联：Unconjugated
产品属性
克隆类型：多克隆抗体
Format：Whole IgG
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH 7.4
防腐剂：0.05% Proclin 300
浓度：见COA
储存温度：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联：Unconjugated
图片
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Western Blot: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - Western Blot
All lanes: PKM2 Antibody (Ab122149) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 61 kDa
Exposure time: 3.7 s
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IF/ICC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cell
Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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Western Blot: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - Western Blot
Lane 1: Wild-type MCF7 cell lysateLane 2: PKM2 knockout MCF7 cell lysatePredicted MW: 56,58 kDaObserved MW: 60 kDaPrimary Ab: 2 µg/mL PKM2 Antibody (Ab122149) Second Ab: 0.2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IF/ICC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IF/ICC
AF488 staining on IF
Samples: HeLa cell
Primary Ab: 20 μg/mL PKM2 Antibody (Ab122149)
Second Ab: 2 μg/mL AF488-Linked Caprine Anti-Rabbit IgG Polyclonal
Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Lung Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Testis Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Oviduct Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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IHC: PKM2 Antibody (Ab122149)
PKM2 Antibody (Ab122149) - IHC
DAB staining on IHC
Samples: Mouse Oviduct Tissue
Primary Ab: 10 µg/mL PKM2 Antibody (Ab122149)
Control: Used PBS instead of PKM2 Antibody (Ab122149)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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