LAMP1 抗体;LAMP1 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P13712(LAMP1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

LAMP1 (lysosomal-associated membrane protein 1) is a transmembrane glycoprotein predominantly localized to the lysosomal membrane, playing critical roles in lysosomal biogenesis, autophagy, and cellular waste degradation. As a member of the LAMP family, it consists of two luminal domains connected by a hinge region, heavily glycosylated to protect lysosomal membranes from enzymatic degradation. LAMP1 antibodies are widely used tools in cell biology and biomedical research to study lysosomal dynamics, organelle integrity, and disease mechanisms.

These antibodies specifically recognize epitopes on the LAMP1 protein, often targeting its luminal domain due to high immunogenicity. They are produced in various host species (e.g., rabbit, mouse) using immunogens like recombinant proteins or synthetic peptides. Applications include immunofluorescence, Western blotting, and immunohistochemistry to visualize lysosomal distribution, monitor autophagy flux, or investigate lysosomal dysfunction in cancers, neurodegenerative disorders (e.g., Alzheimer’s), and lysosomal storage diseases.

Commercial LAMP1 antibodies may vary in clonality (monoclonal/polyclonal), epitope specificity, and cross-reactivity across species (human, mouse, rat). Validation is essential, as nonspecific binding can occur due to homology with LAMP2. Research using LAMP1 antibodies has advanced understanding of cellular homeostasis, pathogen invasion mechanisms (e.g., viruses exploiting lysosomes), and therapeutic targeting of lysosomal pathways.