MBD2-b抗体;MBD2-b Antibody—艾普蒂 新品

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     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P13142(MBD2-b Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The MBD2-b antibody is a specialized tool used to study the methyl-CpG-binding domain protein 2 (MBD2), a key epigenetic regulator involved in DNA methylation-mediated gene silencing. MBD2. a member of the methyl-CpG-binding protein family, recognizes methylated DNA through its conserved methyl-binding domain (MBD) and recruits chromatin-modifying complexes, such as the MeCP1 complex, to promote transcriptional repression. The MBD2 gene undergoes alternative splicing, yielding two isoforms: MBD2-a (full-length) and MBD2-b (shorter isoform lacking the C-terminal region). MBD2-b retains the MBD but lacks domains critical for interaction with chromatin remodelers, potentially altering its functional role compared to MBD2-a.

Antibodies targeting MBD2-b are designed to distinguish this isoform from MBD2-a and other MBD family members. They are widely used in epigenetic research to investigate DNA methylation dynamics, gene regulation, and chromatin remodeling in diseases like cancer, neurological disorders, and developmental abnormalities. MBD2-b has been implicated in tumorigenesis, immune regulation, and stem cell differentiation, though its precise biological functions remain under study. Validated applications for MBD2-b antibodies include Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity validation is crucial, as cross-reactivity with MBD2-a or paralogs like MBD1 or MBD3 could lead to misinterpretation. These antibodies serve as essential tools for unraveling isoform-specific mechanisms in epigenetic silencing pathways.