MBD2抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Human cerebrum tissue and Mouse brain tissue lysates, Primary antibody: P06256(MBD2 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P06256(MBD2 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P06256(MBD2 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The Methyl-CpG Binding Domain Protein 2 (MBD2) is a member of the MBD family, which plays a critical role in interpreting DNA methylation marks to regulate gene expression. MBD2 specifically binds to methylated CpG regions through its conserved methyl-binding domain, facilitating recruitment of chromatin-modifying complexes like the MeCP1 or NuRD complex, leading to transcriptional repression. It is implicated in epigenetic silencing of tumor suppressor genes, stem cell differentiation, and immune regulation.

MBD2 antibodies are essential tools for studying DNA methylation-dependent gene regulation. They are widely used in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, immunofluorescence, and immunohistochemistry to detect MBD2 expression, localization, and interaction partners. Researchers employ these antibodies to explore MBD2's role in diseases, including cancer (where it may act as an oncogene or tumor suppressor depending on context), neurological disorders, and autoimmune conditions.

Most MBD2 antibodies are raised against epitopes within the N-terminal methyl-binding domain or C-terminal regions. Validation often includes knockout (KO) controls to ensure specificity. Recent studies highlight MBD2's involvement in T-cell function and inflammation, expanding its relevance in immunotherapy research. Commercial antibodies are available in monoclonal or polyclonal formats, typically generated in hosts like rabbit or mouse. Understanding MBD2 dynamics through these antibodies continues to advance epigenetic and disease-mechanism studies.