Histone H3K9ac抗体—艾普蒂 新品

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     Microtiter wells were coated with various peptides. P15805 was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the the secondary antibody. Result: P15805 specifically reacts with acetylated H3K9ac.    

  
  

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     Lane 1: 15 µg of MCF-7 lysate;  Lane 2: 15 µg of MCF-7 lysate treated with 1 mM sodium butyrate for 6 hs;  Lane 3: 15 µg of MCF-7 lysate treated with 5 mM sodium butyrate for 6 hs;Lane 4: 15 µg of MCF-7 lysate treated with 10 mM sodium butyrate for 6 hs;  Result: P15805 can detect H3K9ac by Western blotting.    

  
  

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     Lane 1: 15 µg of HeLa lysate;  Lane 2: H3K9ac immunoprecipitated from HeLa lysate by P15805;  Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: P15805 can immunoprecipitate H3K9ac;    

  
  

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     IHC-P analysis in hepatocellular carcinoma tissue by H3K9ac antibody (P15805) Immunohistochemistry was performed using sections of the formalin-fixed paraffin- embedded hepatocellular carcinoma tissue;Result: Vitually all cells are stained at nuclei.    

  
  

Histone H3K9ac (acetylated lysine 9 on histone H3) is a post-translational modification associated with active gene transcription. Acetylation at this site neutralizes the positive charge of lysine, reducing its affinity for the negatively charged DNA backbone. This relaxes chromatin structure, facilitating access for transcriptional machinery and promoting gene expression. The H3K9ac modification is enriched at promoters and enhancers of actively transcribed genes, making it a key epigenetic marker for studying transcriptional regulation.

Antibodies targeting H3K9ac are critical tools in epigenetics research. They are widely used in techniques like chromatin immunoprecipitation (ChIP-seq) to map acetylation patterns genome-wide, as well as in immunoblotting and immunofluorescence to assess spatial and temporal dynamics of chromatin states. These antibodies help investigate how environmental cues, cellular differentiation, or disease states alter epigenetic landscapes. For instance, aberrant H3K9ac levels have been linked to cancer, neurological disorders, and metabolic diseases, underscoring its biological relevance.

Validation of H3K9ac-specific antibodies is essential, as cross-reactivity with other acetylated histone residues (e.g., H3K14ac) could compromise data interpretation. Rigorous testing using knockout models, peptide competition assays, and mass spectrometry is typically required. Commercially available H3K9ac antibodies are often generated using synthetic acetylated peptides as immunogens, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) varying by supplier. Their applications span basic research in chromatin biology to translational studies exploring epigenetic therapies.