MAPK1/MAPK3抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: NIH/3T3 cell, Mouse brain tissue, HepG2, K562 cell lysates, Primary antibody: P10840(MAPK1/MAPK3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 40 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P10840(MAPK1/MAPK3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The mitogen-activated protein kinases MAPK1 (ERK2) and MAPK3 (ERK1) are serine/threonine kinases central to the MAPK signaling cascade, which regulates critical cellular processes such as proliferation, differentiation, survival, and apoptosis. These kinases are activated through a conserved phosphorylation mechanism: upstream signals (e.g., growth factors, cytokines, or stress) trigger a phosphorylation cascade involving Ras, Raf, MEK, and ultimately MAPK1/3. Dual phosphorylation at Thr202/Tyr204 in MAPK1 (Thr185/Tyr187 in MAPK3) induces conformational changes, enabling their translocation to the nucleus to regulate transcription factors like c-Fos and c-Jun.

Antibodies targeting MAPK1/MAPK3 are essential tools for studying their expression, activation, and localization. Commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry, these antibodies fall into two categories: those detecting total MAPK1/3 (regardless of phosphorylation status) and phospho-specific antibodies that recognize the active, dually phosphorylated forms. Such specificity allows researchers to differentiate between basal and activated kinase states, providing insights into pathway dynamics under experimental or pathological conditions.

MAPK1/MAPK3 dysregulation is implicated in cancer, inflammatory diseases, and neurodegenerative disorders, making these antibodies valuable for mechanistic studies and drug discovery. However, users must validate antibody specificity, considering cross-reactivity with related kinases or species variations. Many commercial antibodies are validated using knockout cell lines or peptide blocking assays. Overall, MAPK1/MAPK3 antibodies remain indispensable for dissecting ERK signaling roles in health and disease.