GAD1抗体;GAD1 Antibody—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue, Primary antibody: P11375(GAD1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P11375(GAD1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The GAD1 antibody targets glutamate decarboxylase 1 (GAD1), an enzyme critical for synthesizing gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. GAD1. also known as GAD67. is one of two isoforms (alongside GAD2/GAD65) encoded by distinct genes. It is predominantly expressed in GABAergic neurons and pancreatic β-cells. In the brain, GAD1 converts glutamate to GABA, regulating neuronal excitability and maintaining the balance between inhibition and excitation. Dysregulation of GAD1 expression or activity is implicated in neurological and psychiatric disorders, including epilepsy, schizophrenia, autism, and anxiety.

GAD1 antibodies are widely used in research to identify GABA-producing neurons, map inhibitory circuits, and investigate GABA-related pathologies. In clinical contexts, autoantibodies against GAD isoforms (primarily GAD65) are biomarkers for autoimmune diseases like type 1 diabetes and stiff-person syndrome. However, cross-reactivity between GAD1 and GAD65 antibodies can occur, necessitating careful validation in experimental or diagnostic settings.

Commercial GAD1 antibodies are typically monoclonal or polyclonal, designed for techniques such as Western blot, immunohistochemistry, or immunofluorescence. Specificity is confirmed using knockout controls or isoform-specific peptides. Researchers must distinguish between endogenous GAD1 expression and autoimmune-associated GAD antibodies to avoid misinterpretation. Overall, GAD1 antibodies serve as essential tools for understanding GABAergic function and its role in health and disease.