重组人GAD1/GAD67蛋白-ACROBiosystems百普赛斯

Human GAD1 / GAD67 Protein, His Tag

分子别名（Synonym）

GAD1,GAD67,Glutamate decarboxylase 1

表达区间及表达系统（Source）

Human GAD1, His Tag (GA1-H5543) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Leu 594 (Accession # Q99259-1).

Predicted N-terminus: Met 1

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus

The protein has a calculated MW of 68.8 kDa. The protein migrates as 66 kDa, 90 kDa and >116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>85% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 20 mM Tris, 500 mM Nacl, pH8.5 with glycerol as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

谷氨酸脱羧酶（GAD）是一种催化谷氨酸脱羧为γ氨基丁酸（GABA）的酶，γ氨基丁酸是高级脑区的主要抑制性递质，也是胰岛中推测的旁分泌激素。GAD使用磷酸吡哆醛（PLP）作为辅因子。GAD的两种分子形式（65 kDa和67 kDa）高度保守，均在中枢神经系统、胰岛细胞、睾丸、输卵管和卵巢中表达。这些异构体在大鼠和小鼠的大脑中呈区域性细胞质分布。GAD65是一种ampiphilic，膜锚定蛋白（585 a.a.），负责囊泡GABA的产生。GAD67是细胞质（594 a.a.），似乎负责大量的细胞质GABA产生。