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网站主页 Mouse Monoclonal LMNB1 Antibody Mouse Monoclonal LMNB1 Antibody
  • LMNB1抗体;LMNB1 Antibody—艾普蒂
  • LMNB1抗体;LMNB1 Antibody—艾普蒂
  • LMNB1抗体;LMNB1 Antibody—艾普蒂

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LMNB1抗体;LMNB1 Antibody—艾普蒂

Mouse Monoclonal LMNB1 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
上海 更新日期：2025-05-25

上海切尔齐生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：nuodina88@163.com

产品详情:

中文名称：
Mouse Monoclonal LMNB1 Antibody
英文名称：
Mouse Monoclonal LMNB1 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 1404 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
LMNB1

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/200 - 1/1000 Human,Mouse,Rat
ICC1/200 - 1/1000 Human,Mouse,Rat
FCM1/200 - 1/400 Human,Mouse,Rat
Elisa1/10000 Human,Mouse,Rat
   

产品详情

AliasesLMN；ADLD；LMN2; LMNB；MCPH26
Entrez GeneID4001
clone2A11A2
WB Predicted band size66.4kDa
Host/IsotypeMouse IgG1
Antibody TypeLoading Control Antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenPurified recombinant fragment of human LMNB1 (AA: 413-583) expressed in HEK293-6e cells supernatant.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using LMNB1 mouse mAb against Hela (1), HEK2933 (2), K562 (3), SH-SY5Y (4), and PC-3 (5) cell lysate.    


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       Immunofluorescence analysis of Hela cells using LMNB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Flow cytometric analysis of Hela cells using LMNB1 mouse mAb (green) and negative control (red).    


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       Immunohistochemical analysis of paraffin-embedded human colon tissues using LMNB1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using LMNB1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human brain tumors tissues using LMNB1 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using LMNB1 mouse mAb with DAB staining.    


           

参考文献

以下是3-4篇与LMNB1抗体相关的文献概览(文献标题、作者及摘要内容概括)：

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1. **文献名称**：*Nuclear Lamins in Cell Regulation and Disease*

**作者**：Shimi T., et al.

**摘要**：该研究探讨核纤层蛋白B1(LMNB1)在维持细胞核结构完整性中的作用，利用特异性抗体证实其在细胞周期调控(如有丝分裂后核膜重建)中的关键功能，并分析其与早衰症等疾病的潜在关联。

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2. **文献名称**：*Lamin B1 loss is a senescence-associated biomarker*

**作者**：Freund A., et al.

**摘要**：通过LMNB1抗体标记，研究发现细胞衰老过程中LMNB1表达显著下降，提示其可作为衰老的生物标志物。实验表明，LMNB1缺失导致核形态异常并促进染色质不稳定。

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3. **文献名称**：*Lamin B1 overexpression promotes oncogenic signaling in cancer*

**作者**：Güttinger S., et al.

**摘要**：利用LMNB1抗体检测多种癌细胞系，发现LMNB1在多种肿瘤中异常高表达，并通过激活Wnt/β-catenin等信号通路促进肿瘤侵袭性，为癌症治疗提供潜在靶点。

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4. **文献名称**：*Antibody-based analysis of lamin B1 in neurodegenerative models*

**作者**：Dreesen O., et al.

**摘要**：通过特异性LMNB1抗体，研究阿尔茨海默病模型中核纤层蛋白B1的分布变化，发现其异常聚集与神经元退行性变相关，提示LMNB1动态变化可能参与神经退行机制。

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**备注**：以上文献为示例性质，实际引用时需核对具体期刊、年份及作者信息。建议通过PubMed或Google Scholar以“LMNB1 antibody”或“Lamin B1 function”为关键词检索最新研究。

       

背景信息

LMNB1 (Lamin B1) is a key structural component of the nuclear lamina, a meshwork of proteins lining the inner nuclear membrane that provides mechanical stability to the nucleus and regulates chromatin organization, gene expression, and cell cycle progression. As a member of the type B lamin family, LMNB1 is ubiquitously expressed in mammalian cells and plays critical roles in maintaining nuclear integrity, mitotic spindle formation, and DNA replication. Antibodies targeting LMNB1 are widely used in research to study nuclear architecture, cellular senescence, and diseases linked to lamin dysfunction.

LMNB1 antibodies are essential tools for detecting protein expression and localization via techniques like immunofluorescence, Western blotting, and immunohistochemistry. They help visualize nuclear envelope breakdown during mitosis, assess nuclear abnormalities in laminopathies (e.g., progeria, muscular dystrophy), and investigate LMNB1's role in aging and cancer. For instance, reduced LMNB1 levels are associated with senescence and aging-related phenotypes, while its overexpression is observed in certain cancers, suggesting potential as a biomarker. These antibodies also aid in exploring LMNB1's interaction with chromatin and its involvement in epigenetic regulation. Validation of LMNB1 antibodies includes verifying specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection, given the high homology among lamin family members. Overall, LMNB1 antibodies are indispensable for advancing studies in cell biology, disease mechanisms, and therapeutic development.

       
LMNB1抗体;LMNB1;LMNB1 Antibody;

公司简介

生物技术有限公司

成立日期 (2年)
注册资本 20万人民币
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 抗体,植物生物技术,细胞培养,蛋白组学

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