Anti Active Sar1 Mouse Monoclonal Antibody

Active Sar1 GTP [30 µL] Catalog Number:  CR-M26916 Gene Symbol:  SAR1 Description:   Anti Active Sar1 Mouse Monoclonal Antibody Background:   Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. Immunogen:   Recombinant full length protein of active Sar1a. Applications:   IP, IHC Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;  1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti active Sar1 antibody recognizes active Sar1 of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:

ar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti active Sar1 monoclonal antibody (Cat. No. CR-M26916). Immunoblot was with an anti Sar1 rabbit polyclonal antibody (Cat. No. CR-M21090).Active Sar1 GTP [30 µL] Catalog Number:  CR-M26916 Gene Symbol:  SAR1 Description:   Anti Active Sar1 Mouse Monoclonal Antibody Background:   Small GTPases are a super-family of cellular signaling regulators. Sar1 is a member of these small GTPases. Sar1 functions as a molecular switch to control protein–protein and protein–lipid interactions that direct vesicle budding from the ER. Immunogen:   Recombinant full length protein of active Sar1a. Applications:   IP, IHC Recommended Dilutions:   1 µg for 1~2 mg total cellular proteins for IP;  1:100 dilution for IHC Concentration:   1 mg/ml Host:   mouse Format:   Liquid Clonality:   Monoclonal Isotype:   IgG Purity:   Purified from ascites Storage buffer: Preservative:  no Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Species Reactivity:  Anti active Sar1 antibody recognizes active Sar1 of vertebrates. Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles Immunoprecipitation/Western blot:   Sar1 activation assay. Purified Sar1 proteins were loaded with GDP (lanes 2 and 3) or GTPγS (lanes 4 and 5). Immunoprecipitation was done with the anti active Sar1 monoclonal antibody (Cat. No. CR-M26916). Immunoblot was with an anti Sar1 rabbit polyclonal antibody (Cat. No. CR-M21090).