CD178抗体;CD178 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Immunofluorescence analysis of Hela cells using CD178 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of Jurkat cells using CD178 mouse mAb (green) and negative control (red).    

  
  

CD178. also known as Fas ligand (FasL), is a member of the tumor necrosis factor (TNF) superfamily and plays a critical role in apoptosis and immune regulation. As a type II transmembrane protein, CD178 binds to its receptor Fas (CD95), triggering caspase-dependent programmed cell death. This interaction is essential for maintaining immune homeostasis, particularly in eliminating infected, cancerous, or autoreactive cells. CD178 is predominantly expressed by activated T cells, natural killer (NK) cells, and immune-privileged tissues (e.g., eyes, testes), where it helps enforce tolerance and prevent inflammation.

CD178 antibodies are research tools designed to detect, quantify, or modulate FasL activity. They are widely used in studying apoptosis pathways, immune cell interactions, and diseases linked to dysregulated Fas/FasL signaling, such as autoimmune disorders (e.g., lupus, rheumatoid arthritis), cancer immune evasion, and transplant rejection. Monoclonal anti-CD178 antibodies can block FasL-Fas binding to inhibit apoptosis in vitro, aiding mechanistic studies, while agonist antibodies may induce cell death in therapeutic contexts. However, therapeutic applications remain experimental due to risks like hepatotoxicity or unintended immune activation. In diagnostics, CD178 antibodies help profile FasL expression in tissues or circulating soluble forms, offering insights into disease progression. Validation of these antibodies requires careful specificity testing, as cross-reactivity with other TNF family members can confound results. Overall, CD178 antibodies remain pivotal in unraveling the complex roles of FasL in health and disease. (298 words)