TUBB3抗体;TUBB3 Antibody—艾普蒂

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using TUBB3 mouse mAb against NIH/3T3 (1), Hela (2), and A549 (3) cell lysate.    

  
  

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     Flow cytometric analysis of Hela cells using TUBB3 mouse mAb (green) and negative control (red).    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using TUBB3 mouse mAb with DAB staining.    

  
  

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     Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using TUBB3 mouse mAb with DAB staining.    

  
  

The TUBB3 antibody targets the βIII-tubulin protein, encoded by the TUBB3 gene, which belongs to the β-tubulin family. βIII-tubulin is a neuron-specific isoform predominantly expressed in developing and mature neurons, where it plays critical roles in axonal guidance, neuronal migration, and cytoskeletal dynamics. Unlike other β-tubulins, TUBB3 is associated with specialized microtubule functions in nerve cells, influencing neurogenesis, plasticity, and intracellular transport.

TUBB3 antibodies are widely used in research and diagnostics to identify neuronal populations, assess neurodevelopmental or neurodegenerative conditions, and characterize neural tumors (e.g., neuroblastomas). Overexpression of βIII-tubulin is also linked to aggressive cancer phenotypes and resistance to microtubule-targeting chemotherapeutics, making it a biomarker in oncology. However, TUBB3 mutations are implicated in rare congenital disorders, such as cortical dysplasia and ocular motility defects, highlighting its clinical relevance.

Validation of TUBB3 antibodies requires attention to specificity due to structural homology among tubulin isoforms. Applications include immunohistochemistry, Western blotting, and flow cytometry. Its dual role in neuronal health and disease underscores its importance in both basic neuroscience and translational medicine.