GATA2抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Hela cell lysate, Primary antibody: P05860(GATA2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

GATA2 antibodies are essential tools in studying the GATA2 transcription factor, a critical regulator of hematopoiesis, vascular development, and stem cell maintenance. GATA2. a member of the GATA family of zinc finger proteins, binds to DNA at specific GATA motifs to activate or repress target genes. It is pivotal in the development and function of hematopoietic stem cells (HSCs), endothelial cells, and certain immune cells. Dysregulation of GATA2 is linked to disorders such as myelodysplastic syndromes (MDS), acute myeloid leukemia (AML), and Emberger syndrome (a rare genetic condition causing lymphedema and immunodeficiency).

In research, GATA2 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to detect protein expression, localization, and DNA-binding activity. These antibodies help elucidate GATA2's role in cellular differentiation, its interaction with co-factors, and its involvement in disease pathways. Commercially available antibodies are typically validated for specificity against human, mouse, or rat GATA2 isoforms, though cross-reactivity and batch variability require careful experimental controls.

Recent studies also explore GATA2's dual role as both an oncogene and tumor suppressor, depending on cellular context, making its antibodies vital for cancer research. Additionally, they aid in diagnosing genetic disorders caused by GATA2 mutations. Proper validation using knockout models or siRNA controls is crucial to ensure reliability in experimental outcomes.