H3-5抗体

The H3-5 antibody specifically targets histone H3. a core component of nucleosomes that play a critical role in chromatin structure and gene regulation. Histone H3 undergoes post-translational modifications (e.g., methylation, acetylation) that influence epigenetic mechanisms, including transcriptional activation or repression. The H3-5 variant, part of the H3.3 subfamily, is encoded by the H3F3B gene and is replication-independent, often incorporated into chromatin during DNA repair or transcriptional activity. This antibody is particularly useful in studying epigenetic marks (e.g., H3K4me3. H3K27ac) associated with active promoters or enhancers. It has applications in ChIP-seq, immunofluorescence, and Western blotting to investigate chromatin dynamics in development, differentiation, and diseases like cancer. Research using H3-5 antibodies has advanced understanding of how histone modifications regulate cellular identity, stem cell plasticity, and oncogenic pathways. Its specificity for H3.3 variants also aids in distinguishing replication-dependent and -independent histone deposition mechanisms.