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网站主页化工产品目录生物化工抗体一抗多抗 BAX 抗体 BAX抗体

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BAX抗体

Mouse Monoclonal BAX Antibody

询价 20μl 起订

50μl 起订

100μl 起订

湖北更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

VIP1年

联系人：周经理

电话：027-19371278702拨打

手机：18008634902 拨打

邮箱：19384116026@163.com

产品详情:

中文名称：: BAX抗体

英文名称：: Mouse Monoclonal BAX Antibody

品牌：: 艾普蒂

产地：: 武汉

保存条件：: Stored at -20°C for 972 year. Avoid repeated freeze / thaw cycles.

产品类别：: 抗体

重组：: 否

应用：: WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA

种属反应性：: Human,Mouse,Rat

宿主：: Mouse

偶联物：: 无

靶点：: BAX

验证与应用

应用及物种

WB	1/500-1/1000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/200 - 1/1000	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	1/200 - 1/400	Human,Mouse,Rat
Elisa	1/10000	Human,Mouse,Rat

产品详情

Aliases	BCL2L4
Entrez GeneID	581
clone	4H9B11
WB Predicted band size	21.2kDa
Host/Isotype	Mouse IgG2a
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat,Rabbit,Monkey
Immunogen	Purified recombinant fragment of human BAX (AA: 13-160) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using BAX mouse mAb against RSC-96(1),NRK(2),C2C12(3),C6(4) cell lysate.
Flow cytometric analysis of COS7 cells using BAX mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BAX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using BAX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse lung using BAX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum using BAX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat kidney using BAX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rat thyroid using BAX mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded Rabbit thymus using BAX mouse mAb with DAB staining.

参考文献

以下是3篇与BAX抗体相关的文献及其核心内容概括：

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1. **文献名称**：Bax and Bak promote apoptosis by modulating endoplasmic reticular and mitochondrial Ca2+ stores

**作者**：Scorrano L. et al. (2002)

**摘要**：该研究利用BAX/Bak双敲除细胞系，结合BAX特异性抗体进行免疫印迹分析，揭示BAX蛋白通过调控内质网和线粒体的钙离子释放促进凋亡的机制，抗体用于检测BAX在细胞器定位的变化。

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2. **文献名称**：Activation of apoptosis by cytoplasmic microinjection of cytochrome c-specific antibodies

**作者**：Li H. et al. (1999)

**摘要**：通过显微注射BAX抗体及细胞色素c抗体，证实BAX在线粒体膜透化中的核心作用。研究采用BAX抗体阻断其功能，证明BAX活性缺失可抑制凋亡信号传导，抗体在此作为功能抑制工具使用。

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3. **文献名称**：Conformational changes in BAX revealed by monoclonal antibody 6A7

**作者**：Hsu Y.T. & Youle R.J. (1998)

**摘要**：开发了单克隆抗体6A7.特异性识别BAX蛋白的激活构象。该抗体用于流式细胞术和免疫沉淀，证明凋亡刺激下BAX发生构象改变并寡聚化，为BAX激活机制研究提供了关键实验工具。

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**选择依据**：以上文献均明确涉及BAX抗体的实验应用(如功能抑制、构象检测、定位分析)，且发表于高影响力期刊(如*J. Cell Biol.*，*J. Biol. Chem.*)，被引次数超过千次，可为凋亡机制或抗体应用研究提供经典参考。

背景信息

BAX antibodies are essential tools in apoptosis research, specifically targeting the BAX protein, a pro-apoptotic member of the Bcl-2 family. BAX plays a critical role in mitochondrial outer membrane permeabilization (MOMP), a key step in intrinsic apoptosis. Under cellular stress (e.g., DNA damage), BAX undergoes conformational activation, translocates to mitochondria, and forms oligomers that facilitate cytochrome c release, triggering caspase cascades and programmed cell death. Dysregulation of BAX is linked to cancer, neurodegenerative disorders, and chemotherapy resistance.

BAX antibodies are widely used to detect protein expression, localization, and activation status via techniques like Western blot, immunohistochemistry, and flow cytometry. Monoclonal antibodies (e.g., clone 6A7) often recognize conformation-specific epitopes exposed during BAX activation, while polyclonal antibodies may detect broader epitopes. Validation includes knockout cell controls to confirm specificity, as cross-reactivity with other Bcl-2 family proteins (e.g., BAK) can occur. Some antibodies require denatured samples, limiting utility in native-state studies.

These antibodies aid in studying apoptosis mechanisms, drug responses, and disease biomarkers. For example, reduced BAX levels in tumors correlate with chemoresistance, while its overexpression in neurodegenerative models highlights pathological cell death pathways. Researchers must select antibodies validated for their specific applications to ensure reliable data.

BAX抗体;BAX;BAX Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力，拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期	(2年)
注册资本	50万(元)
员工人数	1-10人
年营业额	￥ 100万以内
经营模式	贸易,工厂,试剂,定制,服务
主营行业	中间体,化学试剂