基本信息 产品详情 公司简介 推荐产品
  • 首页
  • 个人中心
  • 委托采购
  • 采购清单
网站主页 化工产品目录 生物化工 抗体 一抗 其他一抗 BLNK antibody BLNK抗体
  • BLNK抗体
  • BLNK抗体
  • BLNK抗体

1/3

BLNK抗体

Mouse Monoclonal BLNK Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-09

湖北瑞和宁生物科技有限公司

VIP1年
联系人：周经理
电话：027-19371278702拨打
手机：18008634902 拨打
邮箱：19384116026@163.com

产品详情:

中文名称：
BLNK抗体
英文名称：
Mouse Monoclonal BLNK Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 419 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
BLNK

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Mouse,Rat,,Monkey
IF咨询技术 Human,Mouse,Rat,,Monkey
IHC1/200 - 1/1000 Human,Mouse,Rat,,Monkey
ICC1/200 - 1/1000 Human,Mouse,Rat,,Monkey
FCM1/200 - 1/400 Human,Mouse,Rat,,Monkey
Elisa1/10000 Human,Mouse,Rat,,Monkey
   

产品详情

AliasesAGM4; BASH; LY57; SLP65; BLNK-S; SLP-65; MGC111051
Entrez GeneID29760
clone5G9
WB Predicted band size68kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat,,Monkey
ImmunogenPurified recombinant fragment of human BLNK expressed in E. Coli.  
FormulationPurified antibody in PBS with 0.05% sodium azide.

+ +

  •  

     

       Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);    


  •  

     

       Western blot analysis using BLNK mouse mAb against NIH/3T3 (1) and BCBL-1 (2) cell lysate.    


  •  

     

       Western blot analysis using BLNK mouse mAb against CHO3D10(1),COS7(2),L1210(3),C6(4),NIH/3T3(5),PC-12(6),Mouse liver(7) cell lysate.    


  •  

     

       Immunofluorescence analysis of HepG2 cells using BLNK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.    


  •  

     

       Flow cytometric analysis of NIH/3T3 cells using BLNK mouse mAb (green) and negative control (purple).    


  •  

     

       Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using BLNK mouse mAb with DAB staining.    


           

参考文献

以下是关于BLNK抗体的3-4篇参考文献示例(文献为模拟，供参考)：

---

1. **文献名称**: *BLNK: A Central Scaffold Protein in B Cell Receptor Signaling*

**作者**: Fu, C., et al.

**摘要**: 本研究通过BLNK特异性抗体，揭示了BLNK在B细胞受体(BCR)信号中的支架作用。实验表明，BLNK通过招募PLCγ2和Vav等信号分子，调控钙离子流动和细胞骨架重组，是B细胞活化的关键中介。

2. **文献名称**: *Impaired B Cell Development in BLNK-Deficient Mice*

**作者**: Ishiai, M., et al.

**摘要**: 利用BLNK抗体检测基因敲除小鼠模型，发现BLNK缺失导致前B细胞向成熟B细胞分化停滞，证实BLNK在B细胞发育中不可或缺，并影响免疫球蛋白重链的编辑。

3. **文献名称**: *BLNK Mediates Syk-Dependent Signaling Pathways in B Cells*

**作者**: Kurosaki, T., et al.

**摘要**: 通过共免疫沉淀实验(使用BLNK抗体)，阐明BLNK与Syk激酶的结合促进下游MAPK和NF-κB通路的激活，为B细胞增殖和存活提供分子机制依据。

4. **文献名称**: *BLNK Expression Correlates with Autoimmunity Severity*

**作者**: Wang, L., et al.

**摘要**: 在系统性红斑狼疮(SLE)患者中，采用BLNK抗体进行免疫组化分析，发现BLNK在过度活跃的B细胞中高表达，提示其可作为疾病生物标志物或治疗靶点。

---

**注**：以上文献为示例性内容，实际引用时需核实真实文献来源及准确性。建议通过PubMed或Google Scholar以关键词“BLNK antibody”或“BLNK signaling”检索最新研究。

       

背景信息

**Background of BLNK Antibody**

BLNK (B-cell linker protein), also known as SLP-65 or BASH, is a critical adaptor protein in B-cell receptor (BCR) signaling. It is encoded by the *BLNK* gene and primarily expressed in B lymphocytes. BLNK plays a pivotal role in bridging activated BCR-associated kinases (e.g., Syk) to downstream signaling pathways, including PLCγ activation, calcium mobilization, and MAPK/NF-κB regulation. This interaction is essential for B-cell development, differentiation, and immune response coordination.

BLNK deficiency disrupts BCR signal transduction, leading to impaired B-cell maturation and antibody production. Mutations in *BLNK* are linked to agammaglobulinemia, a primary immunodeficiency characterized by low antibody levels and recurrent infections. Additionally, aberrant BLNK expression has been observed in B-cell malignancies, suggesting its role in oncogenic signaling.

BLNK-specific antibodies are vital tools for studying B-cell biology and pathology. They enable detection of BLNK expression, phosphorylation status, and interactions in immune assays (e.g., Western blot, immunoprecipitation). Research applications include investigating immune disorders, leukemia mechanisms, and therapeutic targets for modulating B-cell activity. These antibodies also aid in diagnosing genetic defects affecting BCR signaling pathways.

In summary, BLNK antibodies provide insights into B-cell function, disease mechanisms, and potential immunotherapies, highlighting their importance in both basic and clinical immunology.

       
BLNK抗体;BLNK;BLNK Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

BLNK抗体相关厂家报价

内容声明
official 进入官方账号
拨打电话 立即询价