简单介绍： Fluorescent dyes absorb light at certain wavelengths and in turn emit their fluorescence energy at a higher wavelength. Each dye has a distinct emission spectrum, which can be exploited for multicolor analysis. eBioscience antibodies are available conjugated to a wide variety of fluorochromes.
The tables below lists the properties of the most commonly used fluorochromes in flow cytometry. The eBioscience product number is the prefix used in our catalog numbering system to indicate the corresponding formats of our antibodies. We are continually developing new fluorochrome conjugated antibodies for both flow cytometry and microscopy. Please feel free to contact us at tech@ebioscience.com for more information!
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流式细胞仪633nm激发光荧光染料介绍
产品编号
荧光染料
激发波长(nm)
发射波长 (nm)
荧光强度
使用说明
1=最低荧光强度
5=最高荧光强度
17
Allophycocyanin (APC)
633
660
4
Detected in FL4 on dual laser instruments;, can be used interchangeably with Cy5 or Alexa Fluor® 647
51
Alexa Fluor® 647
633
668
4
Detected in FL4 on dual laser instruments; can be used interchangeably with Cy5 or APC; photostable, making it a great choice for microscopy, in addition to flow cytometry
19
Cy5
633
670
3
Detected in FL4 on dual laser instruments (used interchangeably with APC or Alexa Fluor® 647); good choice for intracellular labeling and microscopy
30
APC-Cy5.5
633
690
3
Detected in appropriate channel based on filter set arrangement; tandem dye, resonance energy transfer from APC molecule to Cy5.5
56
Alexa Fluor® 700
633
723
2
Although not optimally excited by the He-Ne laser, it does produce a bright signal that can be used as a third dye off this laser; please note that emission wavelength requires a distinct set of filters; most instruments will require a 685 LP mirror and 710/20 filter.
47
APC-eFluorTM 780
633
780
2
Appropriate channel for detection based on filter set arrangement of the instrument; more photostable then APC-Cy7; tandem dye, resonance energy transfer from APC molecule to eFluorTM 780, APC-Cy7, APC-Alexa Fluor® 750 and APC-eFluorTM 780 may be used interchangeably.
27
APC-Alexa Fluor®750
633
775
2
Appropriate channel for detection based on filter set arrangement of the instrument; more photostable then APC-Cy7; tandem dye, resonance energy transfer from APC molecule to Alexa Fluor® 750, dim tandem dye. APC-Cy7, APC-Alexa Fluor® 750 and APC-eFluorTM 780 may be used interchangeably.
Excited by the violet laser; eFluorTM 605NCcan be used in combination with eFluorTM450, please make sure to use this conjugate for your brightest antigen/proteins of interest
94
eFluorTM 625NC
405
625
2
Excited by the violet laser; eFluorTM 625NCcan be used in combination with eFluorTM450, please make sure to use this conjugate for your brightest antigen/proteins of interest
95
eFluorTM 650NC
405
650
3
Excited by the violet laser; eFluorTM 650NCcan be used in combination with eFluorTM450 and eFluorTM 605NC, please make sure to use this conjugate for your brightest antigen/proteins of interest.
48
eFluorTM 450
405
450
2
Excited by the violet laser; eFluorTM 450 can be used interchangeably with Pacific Blue®, can be used in combination with eFluorTM605NC or eFluorTM 625NC; please make sure to use this conjugate for your brightest antigen/proteins of interest
52
Alexa Fluor® 405
405
421
1
Extremely dim dye excited by the violet laser; suitable for only the brightest antigens
57
Pacific Blue®
405
455
2
Excited by the violet laser; Pacific Blue® can be used interchangeably with eFluorTM 450, typically a dimmer fluorochrome; please make sure to use this conjugate for your brightest antigen/proteins of interest
-
Alexa Fluor® 430
405
541
1
Can be used as second dye excited by the violet laser
-
Pacific Orange®
405
551
1
Dim and only suitable for densely expressed targets, due to its 551nm emission wavelength the Pacific Orange®dye can be used with Pacific Blue® for multicolor analysis with the violet or UV light source, however eFluorTM 605NC is a better alternative
Cy-ChromeTM is a registered trademark of Becton, Dickinson and Company. Tri-ColorTM is a registered trademark of Caltag Laboratories. Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc., for research use only.
