胡桃苷B|TQ0089|TargetMol

Juglanin
5041-67-8

￥928 1mg 起订

￥2310 5mg 起订

￥3430 10mg 起订

上海更新日期：2024-12-12

TargetMol中国（陶术生物）

VIP3年

联系人：邵小姐

手机：4008200310 拨打

邮箱：marketing@tsbiochem.com

产品详情:

中文名称：: 胡桃苷B

英文名称：: Juglanin

CAS号：: 5041-67-8

品牌：: TargetMol

产地：: 美国

保存条件：: store at low temperature,keep away from moisture,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.33%

产品类别：: 抑制剂

货号：: TQ0089

Product Introduction

Bioactivity

名称	Juglanin
描述	Juglanin is a JNK activator. Juglanin with inflammation and anti-tumor activities. It can induce apoptosis and autophagy on human breast cancer cells.
细胞实验	Breast cancer cells are plated in 96-well plates with a density of 5×10^3 cells/well. After 12 h, the cells are treated with different concentrations of Juglanin (0 to 40 μM) for different periods of time (24 h and 48 h). Then the fresh mixture of MTS and PMS is added and incubated for 4 h at 37°C according to the manufacturer's protocol. A microplate reader is conducted to determine the absorbance at 500 nm, and the IC50 values are assessed with the probit model. Each one is performed in triplicate.
动物实验	Male BALB/c-nude mice, aged 5 weeks, are used. They are maintained under specific pathogen-free conditions and supplied with sterilized food and water. On day 0, about 5×10^6 MCF-7 cells suspended in 0.1 mL PBS are inoculated subcutaneously in the right flank of each mouse (six mice each group). On day 9, when the tumors reach palpable size of around 200 mm3, mice are randomly assigned to three groups and receive daily intraperitoneal injection with 100 μL of vehicle (10% DMSO, 70% Cremophor/ethanol (3: 1), and 20% PBS), and 5 or 10 Juglanin mg/kg of celastrol. Tumor sizes are measured daily to observe dynamic changes in tumor growth. Body weights are also measured daily. After 7 days of drug administration, when the tumors of the control group reach around 1600 mm3, all mice are killed. Tumors are dissected and stored in liquid nitrogen or fixed in formalin for further analysis.
体外活性	Juglanin以剂量依赖和时间依赖的方式抑制乳腺癌细胞的增殖，并对正常细胞的毒性较小。Juglanin导致癌细胞在G2/M期的累积，并相应地降低G0/G1和S期的数量，在MCF-7和SKBR3细胞中均是这种情况。Juglanin上调磷酸化的Chk2、Chk2、磷酸化的Cdc25C、磷酸化的Cdc2、p27、cyclin D的表达，并下调Cdc25C以及Cdc2的水平。对照细胞的凋亡比例可以忽略不计，而暴露于Juglanin 24小时后，会导致MCF-7和SKBR3细胞中凋亡细胞的剂量依赖性增加。Juglanin显著抑制抗凋亡因子Bcl-2的表达，相反，Bad和Bax的表达在Juglanin处理下被上调。Juglanin显著激活caspases，并导致Caspase-9、Caspase-8和Caspase-3的切割。Juglanin启动ROS的生成，并且在高浓度下显著增加。Juglanin还增加了MCF-7和SKBR3细胞中JNK磷酸化的水平。
存储条件	store at low temperature,keep away from moisture,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 20 mg/mL (47.80 mM), Sonification is recommended. Methanol : 16 mg/mL (38.25 mM), Sonication is recommended.
关键字	JNK \| ROS/JNK \| Inhibitor \| G2/M \| arrest \| Apoptosis \| Juglanin \| Autophagy \| phase \| inhibit \| breast cancer
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胡桃苷B|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期	(12年)
注册资本	566.265100万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
经营模式	贸易,工厂,试剂,定制,服务
主营行业	天然产物,生化试剂,分子生物学,分子砌块,生物技术服务