化合物 Ddr1-In-1|T3337|TargetMol

Ddr1-In-1
1449685-96-4

￥333 1mg 起订

￥482 2mg 起订

￥793 5mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 化合物 Ddr1-In-1

英文名称：: Ddr1-In-1

CAS号：: 1449685-96-4

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.84%

产品类别：: 抑制剂

货号：: T3337

Product Introduction

Bioactivity

Name	Ddr1-In-1
Description	DDR1-IN-1 is an effective and specific DDR1 receptor tyrosine kinase inhibitor (IC50: 105 nM), about 3-fold selectivity over DDR2.
Cell Research	Cells are plated in triplicate at a density of 3000 cells per well in 96-well plates and 1500 cells per well in 384-well plates. Compounds of various concentrations are added into plates for 48 hours. Cell viability is determined using the CellTiter-Glo and CCK-8. Both assays are performed according to the manufacturer's instructions. For CellTiter-Glo assay, luminescence is determined in a multi-label reader. For CCK-8 assay, absorbance is measured in a microplate reader at 450 nM. Data are normalized to control group (DMSO) and represented by the mean of at least two independent measurement with standard error <20%. GI50 were calculated using Prism 5.0.(Only for Reference)
Kinase Assay	General procedure for the EC50 test: DDR1 is induced by 2 Gg/ml doxycycline for 48 hrs prior to DDR1 activation by rat tail collagen I. The DDR1 over-expressed U2OS is pre-treated by media containing each concentration of the compound for 1 hr and treated by changing the media to the EC50 test media containing 10 Gg/ml collagen and each concentration of the compound for 2 hrs. Each cells is washed with cold PBS three times and lysed with the lysis buffer (50 mMTris, pH 7.5, 1% Triton X-100, 0.1% SDS, 150 mM NaCl, 5 mM EDTA, 100 mMNaF, 2 mM Na3VO4, 1 mM PMSF, 10 Gg/ml aprotinin, and 10 Gg/ml leupeptin). The activation of DDR1 is quantified by density using program ImageJ to determine EC50 following Western blot using anti-activated human DDR1b (Y513).
In vitro	在U2OS细胞中，DDR1-IN-1通过EC50为86 nM抑制DDR1的基础自磷酸化，并且在没有胶原蛋白刺激的情况下显示出更强的DDR1自磷酸化抑制作用。在一系列具有DDR1功能增益突变和/或过表达的不同癌症细胞株中，DDR1-IN-1在低于10 μM的浓度下不抑制增殖，而GSK2126458增强了DDR1-IN-1的抗增殖活性。[1]
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 93 mg/mL (168.3 mM) Ethanol : 4 mg/mL (7.23 mM), Heating is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble)
Keywords	Discoidin Domain Receptor \| inhibit \| Ddr-1-In-1 \| Inhibitor \| Ddr1 In 1 \| Ddr1In1 \| Ddr1-In-1
Inhibitors Related	DDR Inhibitor \| FGFR1/DDR2 inhibitor 1 \| DDR1-IN-2 \| WRG-28 \| Merestinib \| Sitravatinib \| VU6015929 \| (S)-4-methyl-N-(3-(4-methyl-1H-imidazol-1-yl)-5-(trifluoromethyl)phenyl)-2-(pyrimidin-5-yl)-1,2,3,4-tetrahydroisoquinoline-7-carboxamide \| DDR-TRK-1 \| LCB 03-0110 \| DDR1-IN-4
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Kinase Inhibitor Library \| Fluorochemical Library \| NO PAINS Compound Library \| Anti-COVID-19 Compound Library \| Membrane Protein-targeted Compound Library \| Tyrosine Kinase Inhibitor Library

Ddr1-In-1|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期	(12年)
注册资本	566.265100万人民币
员工人数	100-500人
年营业额	￥ 1亿以上
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主营行业	天然产物,生化试剂,分子生物学,分子砌块,生物技术服务