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网站主页 化工产品目录 生物化工 抑制剂 表观遗传学(Epigenetics) Histone Methyltransferase 抑制剂 BIX 01294三盐酸盐水合物 化合物 BIX 01294 Trihydrochloride
  • 化合物 BIX 01294 Trihydrochloride|T1959|TargetMol

化合物 BIX 01294 Trihydrochloride|T1959|TargetMol

BIX-01294 trihydrochloride
1392399-03-9
￥257 1mg 起订
￥360 2mg 起订
￥640 5mg 起订
上海 更新日期：2024-12-12

TargetMol中国（陶术生物）

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产品详情:

中文名称：
化合物 BIX 01294 Trihydrochloride
英文名称：
BIX-01294 trihydrochloride
CAS号：
1392399-03-9
品牌：
TargetMol
产地：
美国
保存条件：
Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
纯度规格：
99.95%
产品类别：
抑制剂
货号：
T1959

Product Introduction

Bioactivity

名称BIX-01294 trihydrochloride
描述BIX-01294 trihydrochloride is an inhibitor of G9a histone methyltransferase.In a cell-free assay, the IC50=2.7 μM for G9a histone methyltransferase.
激酶实验ATR for use in the in vitro enzyme assay is obtained from HeLa nuclear extract by immunoprecipitation with rabbit polyclonal antiserum raised to amino acids 400-480 of ATR contained in the following buffer: 25 mM HEPES (pH 7.4), 2 mM MgCl2, 250 mM NaCl, 0.5 mM EDTA, 0.1 mM Na3VO4, 10% v/v glycerol, and 0.01% v/v Tween 20. ATR-antibody complexes are isolated from nuclear extract by incubating with protein A-Sepharose beads for 1 h and then through centrifugation to recover the beads. In the well of a 96-well plate, 10 μL ATR-containing Sepharose beads are incubated with 1 μg of substrate glutathione?S-transferase-p53N66 (NH2-terminal 66 amino acids of p53 fused to glutathione?S-transferase are expressed in?E. coli) in ATR assay buffer (50 mM HEPES (pH 7.4), 150 mM NaCl, 6 mM MgCl2, 4 mM MnCl2, 0.1 mM Na3VO4, 0.1 mM DTT, and 10% (v/v) glycerol) at 37°C in the presence or absence of inhibitor. After 10 min with gentle shaking, ATP is added to a final concentration of 3 μM and the reaction continued at 37°C for an additional 1 h. The reaction is stopped by addition of 100 μL of PBS, and the reaction is transferred to a white opaque glutathione coated 96-well plate and incubated overnight at 4°C. This plate is then washed with PBS/0.05% (v/v) Tween 20, blotted dry, and analyzed by a standard ELISA technique with a phosphoserine 15 p53 antibody. The detection of phosphorylated glutathione?S-transferase-p53N66 substrate is performed in combination with a goat anti-mouse horseradish peroxidase-conjugated secondary antibody. Enhanced chemiluminescence solution is used to produce a signal, and chemiluminescent detection is carried out via a TopCount plate reader. The resulting calculated % enzyme activity is then used to determine the IC50?values for the compounds (IC50?taken as the concentration at which 50% of the enzyme activity is inhibited).
体内活性在野生型ES细胞,小鼠胚胎成纤维细胞和HeLa细胞中, BIX-01294（4.1 μM ）降低H3K9me2水平。BIX-01294能够特异性抑制G9a（IC50=1.7 μM ） 和GLP（IC50=38 μM）。
存储条件Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
溶解度H2O : 60 mg/mL (100 mM)
DMSO : 40 mg/mL (66.66 mM), Sonication is recommended.
关键字SUV39H1(H320R) | BIX-01294 trihydrochloride | p21 | recurrent | BIX-01294 Trihydrochloride | Cdkn1a | MLKL | H3K9me3 | inhibit | Histone Methyltransferase | PRMT1 | p53 | Autophagy | Inhibitor | H3K9me2 | BIX01294 | BIX 01294 trihydrochloride | BIX-01294 | BIX 01294 Trihydrochloride | BIX01294 Trihydrochloride | Gadd45a | BIX01294 trihydrochloride
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BIX 01294|TargetMol

公司简介

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。 凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

成立日期 (12年)
注册资本 566.265100万人民币
员工人数 100-500人
年营业额 ￥ 1亿以上
经营模式 贸易,工厂,试剂,定制,服务
主营行业 天然产物,生化试剂,分子生物学,分子砌块,生物技术服务

化合物 BIX 01294 Trihydrochloride相关厂家报价

  • BIX-01294
  • BIX-01294
  • 爱必信（上海）生物科技有限公司
  • 2023-10-16
  • ￥1926
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