化合物 MM-102|T6333|TargetMol

MM-102
1417329-24-8

￥428 1mg 起订

￥622 2mg 起订

￥1280 5mg 起订

上海更新日期：2024-09-29

TargetMol中国（陶术生物）

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产品详情:

中文名称：: 化合物 MM-102

英文名称：: MM-102

CAS号：: 1417329-24-8

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.08%

产品类别：: 抑制剂

货号：: T6333

Product Introduction

Bioactivity

Name	MM-102
Description	MM-102 (HMTase Inhibitor IX) is a potent inhibitor of WDR5/MLL interaction with IC50 of 2.4 nM and Ki of less than 1 nM in a WDR5 binding assay. MM-102 can also specifically inhibit cell growth and induce apoptosis in leukemia cells carrying MLL1 fusion protein.
Cell Research	MV4;11, KOPN8, and K562 cells are cultured in RPMI 1640 medium (ATCC) supplemented with 10% fetal bovine serum and 100 U/L penicillin-streptomycin and incubated at 37 °C under 5% CO2. Cells are seeded into 12-well plates for suspension at a density of 5 × 105 per well (1 mL) and treated with either vehicle control (DMSO, 0.2%) or MM-102 for 7 days. The medium is changed every 2 days, and compounds are resupplied. The CellTiter-Glo Luminescent Cell Viability Assay kit is used following the manufacturer’s instruction. First, 100 μL of the assay reagent is added into each well, and the content is mixed for 2 min on an orbital shaker to induce cell lysis. After 10 min incubation at room temperature, the luminescence is read on a microplate reader. (Only for Reference)
Kinase Assay	In Vitro Histone Methyltransferase (HMT) Assay: The HMT assay is performed in 50 mM HEPES pH 7.8, 100 mM NaCl, 1.0 mM EDTA, and 5% glycerol at 22 °C. Each reaction contains 1.5 μCi of the co-factor, 3H-S-adenosylmethionine. H3 10-residue peptide is used as the substrate at 50 μM. Compounds are added at concentrations ranging from 0.125 to 128 μM and incubated with the pre-assembled WDR5/RbBP5/ASH2L complex at a final concentration of 0.5 μM for each protein for 2–5 min. Reactions are initiated by addition of the MLL1 protein at a final concentration of 0.5 μM and allowed to proceed for 30 min before preparing scintillation counting. To count samples, reactions are spotted on separate squares of P81 filter paper and precipitated by submerging in freshly prepared 50 mM sodium bicarbonate buffer with pH 9.0. After washing and drying, samples are vortexed in Ultima Gold scintillation fluid and counted. As a negative control, assays are performed using 0.5 μM MLL1/WDR5/RbBP5/ASH2L complex assembled with the non-interacting mutant, WDR5D107A.
In vitro	方法：用 MM-102 (HMTase Inhibitor IX) （25,50μM）处理 MLL1-AF9 转导的小鼠细胞，然后用定量 RT-PCR （QRT-PCR）进行基因表达分析。结果：MM-102 以剂量依赖性方式降低 HoxA9 mRNA 的表达 MM-102 在 50 μM 时 Meis-1 表达明显下降，MM-102不影响管家基因GAPDH的表达。[1] 方法：用 20 μM MM-102（20 μM ，20小时）预处理细胞 24 小时，然后用 500 μM SNP 孵育，约24小时后评估细胞凋亡活性。结果：SNP显着刺激软骨细胞凋亡，而用MM-102预处理的细胞减轻了SNP刺激软骨细胞凋亡，MM-102预处理有效挽救了FSS对软骨细胞的负面影响，这可能为基于表观遗传学的OA治疗奠定基础。[2]
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
Solubility Information	DMSO : 93 mg/mL (138.8 mM) H2O : 92 mg/mL (137.4 mM) Ethanol : 93 mg/mL (138.8 mM)
Keywords	MM 102 \| Inhibitor \| MM-102 \| inhibit \| Histone Methyltransferase
Inhibitors Related	Tazemetostat hydrobromide \| XY1 \| BML-278 \| MRTX-1719 \| MAK683 \| GSK126 \| Tazemetostat \| EPZ015666 \| UNC 0631 \| AMI-1 free acid \| MS37452 \| Piribedil
Related Compound Libraries	Inhibitor Library \| Bioactive Compound Library \| Bioactive Compounds Library Max \| Histone Modification Compound Library \| Epigenetics Compound Library \| PPI Inhibitor Library

MM102|||HMTase Inhibitor IX|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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