靛玉红-3' -单肟|T5200|TargetMol

Indirubin-3'-monoxime
160807-49-8

￥186 1mg 起订

￥413 5mg 起订

￥662 10mg 起订

上海更新日期：2024-09-29

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产品详情:

中文名称：: 靛玉红-3' -单肟

英文名称：: Indirubin-3'-monoxime

CAS号：: 160807-49-8

品牌：: TargetMol

产地：: 美国

保存条件：: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

纯度规格：: 99.65%

产品类别：: 抑制剂

货号：: T5200

Product Introduction

Bioactivity

名称	Indirubin-3'-monoxime
描述	Indirubin-3'-monoxime (Indirubin-3'-oxime) is a potent inhibitor of GSK3β (IC50: 22 nM) and also inhibits CDKs ( (IC50s: 100/180/250 nM for Cdk5/p35, Cdk1/cyclin B, Cdk2/cyclin E).
细胞实验	To determine the effects of aminopurvalanol and indirubin-3′-monoxime on tau phosphorylation, Sf9 cells infected with baculovirus expressing htau23 protein were treated 36 h post-infection (when cells have already expressed levels of tau sufficient for the outgrowth of cell processes) with 20 μM inhibitors for 3 h before being harvested [1].
激酶实验	Kinase activities were assayed in Buffer A or C (unless otherwise stated), at 30?°C, at a final ATP concentration of 15 μM. Blank values were subtracted, and activities were calculated as picomoles of phosphate incorporated for a 10-min incubation. The activities are usually expressed in percentage of the maximal activity, i.e. in the absence of inhibitors. Controls were performed with appropriate dilutions of dimethyl sulfoxide. In a few cases, phosphorylation of the substrate was assessed by autoradiography after SDS-PAGE [1].
动物实验	Male mice (5–6 weeks old) were randomly assigned into five groups (n = 10). Group 1: received normal pellet diet (NPD); Group 2: received a HFD; Group 3–5 received HFD for 8 weeks followed by Indirubin-3'-monoxime (IMX) treatment (0.1, 0.2 and 0.4 mg/kg i.p, respectively) once daily for 1 week. IMX was dissolved in (2.5% v/v) DMSO in saline. The mice in NPD and HFD groups received an equivalent volume of vehicle (2.5% v/v DMSO in saline). The composition of HFD was similar as described by Srinivasan. Doses of IMX were selected based on the reports available in literature. Mice were kept under standard husbandry conditions (22 ± 1 C and 60% humidity) and maintained on a 12/12-h light/dark schedule with free access to food and water for 8 weeks. Body weight was recorded weekly throughout the experimental period [4].
体外活性	Indirubins是强效的GSK-3 beta抑制剂（IC50：5-50 nM）。通过GSK-3β，细菌表达的重组人tau蛋白确实在体外被磷酸化，且这种磷酸化作用可被Indirubin-3'-monoxime 以剂量依赖的方式抑制，IC50值约为100 nM [1]。Indirubin-3'-monoxime可可逆性地在G2期阻滞异步HBL-100细胞。它抑制了共识CDK磷酸化位点以及在特定CDK1/cyclin B磷酸化位点上的核仁蛋白的磷酸化 [2]。在基于细胞和无细胞的实验中，Indirubin-3'-monoxime选择性地抑制了5-脂氧合酶（5-LO），这是LT生物合成中的关键酶，其IC50值处于低μM范围内 [3]。
体内活性	经IMX处理的小鼠，在血浆中的葡萄糖、甘油三酯、胆固醇、胰岛素水平方面显示出显著下降，并在学习记忆能力上表现出改善；同时减轻了氧化应激和AChE活性。此外，IMX还能剂量依赖性地提高高脂饮食（HFD）喂养小鼠大脑中的胰岛素和BDNF水平[4]。
存储条件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : Insoluble Ethanol : 15 mg/mL DMSO : 55 mg/mL (198.36 mM)
关键字	Indirubin 3' monoxime \| Lipoxygenase \| GSK-3 \| LOX \| inhibit \| Indirubin3'monoxime \| Inhibitor \| CDK \| Glycogen synthase kinase-3 \| Glycogen synthase kinase 3 \| Indirubin-3'-monoxime \| Cyclin dependent kinase
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相关库	抑制剂库 \| 神经保护化合物库 \| 经典已知活性库 \| 已知活性化合物库 \| 抗癌细胞代谢库 \| 细胞周期化合物库 \| 激酶抑制剂库 \| 抗衰老化合物库 \| 抗氧化化合物库 \| 神经退行性疾病化合物库

靛玉红-3' -单肟|||Indirubin-3'-oxime|TargetMol

TargetMol Chemicals Inc. 总部位于马萨诸塞州波士顿，致力于为全球生化领域科学家的研究提供专业的产品和服务。TargetMol?品牌的客户群分布于40多个国家和地区，已发展成为全球知名的化合物库和小分子化合物研究供应商。 TargetMol?可提供160多种满足不同需求的化合物库，以及多种类型的生化试剂产品，包括12000多种抑制剂、16000多种天然产物和各类多肽、抗体、生命科学试剂盒等，此外，我们还建设有CADD（计算机辅助药物设计）研究中心、药理实验室、药化合成平台三大技术中心，全方位满足客户的定制需求。凭借我们优质的产品和服务、快速高效的全球供应链和专业的技术支持，我们将有效帮助您缩短研发周期，取得更成功的结果。

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